SMC3 Polyclonal antibody
SMC3 Polyclonal Antibody for WB, IF, FC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, FC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 14185-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, Jurkat cells, mouse brain tissue |
Positive IF detected in | HeLa cells |
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2400 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 1 publications below |
CoIP | See 1 publications below |
Product Information
14185-1-AP targets SMC3 in WB, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | SMC3 fusion protein Ag5440 相同性解析による交差性が予測される生物種 |
Full Name | structural maintenance of chromosomes 3 |
Calculated molecular weight | 142 kDa |
Observed molecular weight | 140 kDa |
GenBank accession number | BC047324 |
Gene symbol | SMC3 |
Gene ID (NCBI) | 9126 |
RRID | AB_2192625 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for SMC3 antibody 14185-1-AP | Download protocol |
IF protocol for SMC3 antibody 14185-1-AP | Download protocol |
FC protocol for SMC3 antibody 14185-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Biology (Basel) In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome. |