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Validation Data Gallery

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  • Western Blot (WB) analysis of RAW264.7 using SMARCD2 Polyclonal antibody (11156-1-AP)

    WB analysis of RAW264.7 using 11156-1-AP (same clone as 11156-1-PBS)

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 11156-1-AP (SMARCD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11156-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse thymus tissue using SMARCD2 Polyclonal antibody (11156-1-AP)

    WB analysis of mouse thymus using 11156-1-AP (same clone as 11156-1-PBS)

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11156-1-AP (SMARCD2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11156-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using SMARCD2 Polyclonal antibody (11156-1-AP)

    IHC staining of human cervical cancer using 11156-1-AP (same clone as 11156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11156-1-AP (SMARCD2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11156-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat spleen tissue using SMARCD2 Polyclonal antibody (11156-1-AP)

    IHC staining of rat spleen using 11156-1-AP (same clone as 11156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 11156-1-AP (SMARCD2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11156-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat spleen tissue using SMARCD2 Polyclonal antibody (11156-1-AP)

    IHC staining of rat spleen using 11156-1-AP (same clone as 11156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 11156-1-AP (SMARCD2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11156-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using SMARCD2 Polyclonal antibody (11156-1-AP)

    IHC staining of mouse spleen using 11156-1-AP (same clone as 11156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 11156-1-AP (SMARCD2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11156-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using SMARCD2 Polyclonal antibody (11156-1-AP)

    IHC staining of mouse spleen using 11156-1-AP (same clone as 11156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 11156-1-AP (SMARCD2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11156-1-PBS in a different storage buffer formulation.

SMARCD2 Polyclonal antibody, PBS Only

SMARCD2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 11156-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11156-1-PBS targets SMARCD2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1630

Product name: Recombinant human SMARCD2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-78 aa of BC018953

Sequence: RDFMLSFSTDPQDFIQEWLRSQRRDLKIITDVIGNPEEERRAAFYHQPWAQEAVGRHIFAKVQQRRQELEQVLGIRLT

相同性解析による交差性が予測される生物種
Full Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 2
Calculated molecular weight 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC018953
Gene Symbol SMARCD2
Gene ID (NCBI) 6603
RRIDAB_2192145
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92925
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The SWI/SNF chromatin remodelling complexes are important regulators of transcription; they consist of large multisubunit assemblies containing either Brm or Brg1 as the catalytic ATPase subunit and a variable subset of approximately 10 Brg/Brm-associated factors (BAF) [PMID:20148946]. SMARCD2 is one of BAF60 proteins, which are found in most complexes, is thought to bridge interactions between transcription factors and SWI/SNF complexes. [PMID:9427560]

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