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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using SMARCD1 Polyclonal antibody (10998-2-AP)

    WB analysis of mouse brain using 10998-2-AP (same clone as 10998-2-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10998-2-AP (SMARCD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10998-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using SMARCD1 Polyclonal antibody (10998-2-AP)

    WB analysis of mouse brain using 10998-2-AP (same clone as 10998-2-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10998-2-AP (SMARCD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10998-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using SMARCD1 Polyclonal antibody (10998-2-AP)

    WB analysis of Jurkat using 10998-2-AP (same clone as 10998-2-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10998-2-AP (SMARCD1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10998-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using SMARCD1 Polyclonal antibody (10998-2-AP)

    WB analysis of human brain using 10998-2-AP (same clone as 10998-2-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 10998-2-AP (SMARCD1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10998-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using SMARCD1 Polyclonal antibody (10998-2-AP)

    IP experiment of mouse brain using 10998-2-AP (same clone as 10998-2-PBS)

    IP result of anti-SMARCD1 (IP:10998-2-AP, 3ug; Detection:10998-2-AP 1:600) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 10998-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using SMARCD1 Polyclonal antibody (10998-2-AP)

    IHC staining of human lymphoma using 10998-2-AP (same clone as 10998-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 10998-2-AP (SMARCD1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10998-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using SMARCD1 Polyclonal antibody (10998-2-AP)

    IF Staining of SH-SY5Y using 10998-2-AP (same clone as 10998-2-PBS)

    Immunofluorescent analysis of SH-SY5Y cells, using SMARCD1 antibody 10998-2-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 10998-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SMARCD1 Polyclonal antibody, PBS Only

SMARCD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 10998-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10998-2-PBS targets SMARCD1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1453

Product name: Recombinant human SMARCD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 126-476 aa of BC009368

Sequence: RLDIQEALKRPIKQKRKLRIFISNTFNPAKSDAEDGEGTVASWELRVEGRLLEDSALSKYDATKQKRKFSSFFKSLVIELDKDLYGPDNHLVEWHRTATTQETDGFQVKRPGDVNVRCTVLLMLDYQPPQFKLDPRLARLLGIHTQTRPVIIQALWQYIKTHKLQDPHEREFVICDKYLQQIFESQRMKFSEIPQRLHALLMPPEPIIINHVISVDPNDQKKTACYDIDVEVDDTLKTQMNSFLLSTASQQEIATLDNKIHETIETINQLKTQREFMLSFARDPQGFINDWLQSQCRDLKTMTDVVGNPEEERRAEFYFQPWAQEAVCRYFYSKVQQRRQELEQALGIRNT

相同性解析による交差性が予測される生物種
Full Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1
Calculated molecular weight 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC009368
Gene Symbol SMARCD1
Gene ID (NCBI) 6602
RRIDAB_2192133
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96GM5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SMARCD1 belongs to the nuclear SWI/SNF chromatin remodeling complex, which regulates transcription and interacts with nucleolin in the nucleus. In addition, it's a member of the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). It possesses a SWIB domain which is homologous to and shares a common fold with the p53-binding domain of MDM2, a cell cycle regulator which interacts with NPM1. SMARCD1 has a strong influence on the Vitamin D-mediated transcriptional activity from an enhancer Vitamin D receptor element (VDRE). And it can mediates critical interactions between nuclear receptors and the BRG1/SMARCA4 chromatin-remodeling complex for transactivation. This antibody may have across reaction with SMARCD2.

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