Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, A431 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human medulloblastoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IP | See 1 publications below |
Product Information
12018-1-AP targets BAF170 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | BAF170 fusion protein Ag2634 相同性解析による交差性が予測される生物種 |
| Full Name | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2 |
| Calculated molecular weight | 1214 aa, 132 kDa |
| Observed molecular weight | 170 kDa |
| GenBank accession number | BC013045 |
| Gene Symbol | BAF170 |
| Gene ID (NCBI) | 6601 |
| RRID | AB_2192010 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TAQ2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BAF170, also named as SWI/SNF complex 170 kDa subunit or SMARCC2, is a 1214 amino acid protein, which belongs to the SMARCC family. BAF170 is ubiquitously expressed. BAF170 is involved in transcriptional activation and repression of select genes by chromatin remodeling. BAF170 may be required for CoREST dependent repression of neuronal specific gene promoters in non-neuronal cells. BAF170 belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). The calcualted molecular weight of BAF170, is 133 kDa, but modified BAF170 is about 170 kDa. (PMID: 8804307)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BAF170 antibody 12018-1-AP | Download protocol |
| IHC protocol for BAF170 antibody 12018-1-AP | Download protocol |
| IF protocol for BAF170 antibody 12018-1-AP | Download protocol |
| IP protocol for BAF170 antibody 12018-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell SPT5 stabilizes RNA polymerase II, orchestrates transcription cycles, and maintains the enhancer landscape. | ||
Aging (Albany NY) FBXO28 promotes proliferation, invasion, and metastasis of pancreatic cancer cells through regulation of SMARCC2 ubiquitination | ||
Biochem Biophys Res Commun SMARCC2 silencing suppresses oncogenic activation through modulation of chromatin accessibility in breast cancer
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