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- KD/KO Validated
SMARCA5 Polyclonal antibody
SMARCA5 Polyclonal Antibody for WB, IF, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IF, ELISA
Conjugate
Unconjugated
Cat no : 13066-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF) | IF : 1:500-1:2000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
Product Information
13066-1-AP targets SMARCA5 in WB, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SMARCA5 fusion protein Ag3759 相同性解析による交差性が予測される生物種 |
| Full Name | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5 |
| Calculated molecular weight | 1052 aa, 122 kDa |
| Observed molecular weight | 122 kDa |
| GenBank accession number | BC023144 |
| Gene symbol | SMARCA5 |
| Gene ID (NCBI) | 8467 |
| RRID | AB_2191854 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SMARCA5, also named as SNF2H or WCRF135, is a 1052 amino acid protein, which localizes in nucleus and is overexpressed in CD34-positive erythrocyte progenitor cells in acute myeloid leukemia. SMARCA5 is one of the most conserved chromatin remodeling factors. It exists in a variety of oligosubunit complexes that move DNA with respect to the histone octamer to generate regularly spaced nucleosomal arrays. Smarca5 interacts with different accessory proteins and represents a molecular motor for DNA replication, repair, and transcription.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SMARCA5 antibody 13066-1-AP | Download protocol |
| IF protocol for SMARCA5 antibody 13066-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Exp Med Nuclear DEK preserves hematopoietic stem cells potential via NCoR1/HDAC3-Akt1/2-mTOR axis. | ||
Nucleic Acids Res SIRT6 coordinates with CHD4 to promote chromatin relaxation and DNA repair.
| ||
Arterioscler Thromb Vasc Biol Novel Long Noncoding RNA, Macrophage Inflammation-Suppressing Transcript (MIST), Regulates Macrophage Activation During Obesity. |
