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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMARCA4 Recombinant antibody (83310-2-RR)

    WB analysis using 83310-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83310-2-RR (SMARCA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using SMARCA4 Recombinant antibody (83310-2-RR)

    IHC staining of mouse colon using 83310-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with .

  • Immunohistochemistry (IHC) staining of mouse colon tissue using SMARCA4 Recombinant antibody (83310-2-RR)

    IHC staining of mouse colon using 83310-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with .

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using SMARCA4 Recombinant antibody (83310-2-RR)

    IHC staining of human tonsillitis using 83310-2-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using SMARCA4 Recombinant antibody (83310-2-RR)

    IHC staining of human tonsillitis using 83310-2-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using SMARCA4 Recombinant antibody (83310-2-RR)

    IHC staining of human placenta using 83310-2-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using SMARCA4 Recombinant antibody (83310-2-RR)

    IHC staining of human placenta using 83310-2-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using SMARCA4 Recombinant antibody (83310-2-RR)

    IHC staining of mouse cerebellum using 83310-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using SMARCA4 Recombinant antibody (83310-2-RR)

    IHC staining of mouse cerebellum using 83310-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using SMARCA4 Recombinant antibody (83310-2-RR)

    IHC staining of mouse lung using 83310-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using SMARCA4 Recombinant antibody (83310-2-RR)

    IHC staining of mouse lung using 83310-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using SMARCA4 Recombinant antibody (83310-2-RR)

    IHC staining of rat cerebellum using 83310-2-RR

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using SMARCA4 Recombinant antibody (83310-2-RR)

    IHC staining of rat cerebellum using 83310-2-RR

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat colon tissue using SMARCA4 Recombinant antibody (83310-2-RR)

    IHC staining of rat colon using 83310-2-RR

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat colon tissue using SMARCA4 Recombinant antibody (83310-2-RR)

    IHC staining of rat colon using 83310-2-RR

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat lung tissue using SMARCA4 Recombinant antibody (83310-2-RR)

    IHC staining of rat lung using 83310-2-RR

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat lung tissue using SMARCA4 Recombinant antibody (83310-2-RR)

    IHC staining of rat lung using 83310-2-RR

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SMARCA4 Recombinant antibody (83310-2-RR)

    IF Staining of HepG2 using 83310-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMARCA4 antibody (83310-2-RR, Clone: 240172F4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of Jurkat cells using SMARCA4 Recombinant antibody (83310-2-RR)

    FC experiment of Jurkat using 83310-2-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug SMARCA4 Recombinant antibody (83310-2-RR, Clone:240172F4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83310-2-RR

    Affinity and Kinetic Characterization of 83310-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83310-2-RR against Human SMARCA4 were performed. The affinity constant is 3.66 nM.

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SMARCA4 Recombinant antibody

SMARCA4 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83310-2-RR
Clone No.240172F4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

240172F4, ATP dependent helicase SMARCA4, ATP-dependent helicase SMARCA4, BAF190, BAF190A

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, HSC-T6 cells, NIH/3T3 cells, HEK-293T cells, C6 cells
Positive IHC detected inhuman tonsillitis tissue, human placenta tissue, mouse cerebellum tissue, mouse colon tissue, mouse lung tissue, rat cerebellum tissue, rat colon tissue, rat lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83310-2-RR targets SMARCA4 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SMARCA4 fusion protein Ag16256 相同性解析による交差性が予測される生物種
Full Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Calculated molecular weight 1647 aa, 185 kDa
Observed molecular weight 185 kDa
GenBank accession numberBC150298
Gene Symbol SMARCA4
Gene ID (NCBI) 6597
RRIDAB_3670976
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP51532
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SMARCA4, also named as BAF190A, BRG1, SNF2B and SNF2L4, belongs to the SNF2/RAD54 helicase family. SMARCA4 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It is a component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.

Protocols

Product Specific Protocols
WB protocol for SMARCA4 antibody 83310-2-RRDownload protocol
IHC protocol for SMARCA4 antibody 83310-2-RRDownload protocol
IF protocol for SMARCA4 antibody 83310-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols