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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMARCA2-Specific Recombinant antibody (85018-4-RR)

    WB analysis using 85018-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85018-4-RR (SMARCA2-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using SMARCA2-Specific Recombinant antibody (85018-4-RR)

    IHC staining of human placenta using 85018-4-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85018-4-RR (SMARCA2-Specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SMARCA2-Specific Recombinant antibody (85018-4-RR)

    IHC staining of mouse brain using 85018-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85018-4-RR (SMARCA2-Specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SMARCA2-Specific Recombinant antibody (85018-4-RR)

    IHC staining of mouse brain using 85018-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85018-4-RR (SMARCA2-Specific antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SMARCA2-Specific Recombinant antibody (85018-4-RR)

    IF Staining of HeLa using 85018-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SMARCA2-Specific antibody (85018-4-RR, Clone: 242511E1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 85018-4-RR

    Affinity and Kinetic Characterization of 85018-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85018-4-RR against Human SMARCA2-Specific were performed. The affinity constant is 3.86 nM.

SMARCA2-Specific Recombinant monoclonal antibody

SMARCA2-Specific Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 85018-4-RR
Clone No.242511E1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

SMARCA2, 242511E1, ATP-dependent helicase SMARCA2, BAF190, BAF190B

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, U-251 cells, MCF-7 cells, K-562 cells, NIH/3T3 cells
Positive IHC detected inmouse brain tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85018-4-RR targets SMARCA2-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag25157

Product name: Recombinant human SMARCA2-Specific protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-272 aa of NM_003070

Sequence: MSTPTDPGAMPHPGPSPGPGPSPGPILGPSPGPGPSPGSVHSMMGPSPGPPSVSHPMPTMGSTDFPQEGMHQMHKPIDGIHDKGIVEDIHCGSMKGTGMRPPHPGMGPPQSPMDQHSQGYMSPHPSPLGAPEHVSSPMSGGGPTPPQMPPSQPGALIPGDPQAMSQPNRGPSPFSPVQLHQLRAQILAYKMLARGQPLPETLQLAVQGKRTLPGLQQQQQQQQQQQQQQQQQQQQQQQPQQQPPQPQTQQQQQPALVNYNRPSGPGPELSGP

相同性解析による交差性が予測される生物種
Full Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
Calculated molecular weight 181 kDa
Observed molecular weight210 kDa
GenBank accession numberNM_003070
Gene Symbol SMARCA2
Gene ID (NCBI) 6595
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP51531
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SMARCA2 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. SMARCA2 is involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.

Protocols

Product Specific Protocols
IF protocol for SMARCA2-Specific antibody 85018-4-RRDownload protocol
WB protocol for SMARCA2-Specific antibody 85018-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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