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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMAP2 Recombinant monoclonal antibody (88105-1-RR)

    WB analysis using 88105-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 88105-1-RR (SMAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity of 88105-1-RR

    Affinity of 88105-1-RR

    Biolayer interferometry (BLI) kinetic assays of 88105-1-RR against Human SMAP2 were performed. The affinity constant is 2.41 nM.

SMAP2 Recombinant monoclonal antibody

SMAP2 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 88105-1-RR
Clone No.260364G2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

small ArfGAP2, SMAP1L, Stromal membrane-associated protein 1-like, Stromal membrane-associated protein 2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, PC-3 cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

88105-1-RR targets SMAP2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag9751

Product name: Recombinant human SMAP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 77-429 aa of BC021133

Sequence: QIQCMQEMGNGKANRLYEAYLPETFRRPQIDPAVEGFIRDKYEKKKYMDRSLDINAFRKEKDDKWKRGSEPVPEKKLEPVVFEKVKMPQKKEDPQLPRKSSPKSTAPVMDLLGLDAPVACSIANSKTSNTLEKDLDLLASVPSPSSSGSRKVVGSMPTAGSAGSVPENLNLFPEPGSKSEEIGKKQLSKDSILSLYGSQTPQMPTQAMFMAPAQMAYPTAYPSFPGVTPPNSIMGSMMPPPVGMVAQPGASGMVAPMAMPAGYMGGMQASMMGVPNGMMTTQQAGYMAGMAAMPQTVYGVQPAQQLQWNLTQMTQQMAGMNFYGANGMMNYGQSMSGGNGQAANQTLSPQMWK

相同性解析による交差性が予測される生物種
Full Name small ArfGAP2
Calculated molecular weight 429 aa, 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC021133
Gene Symbol SMAP2
Gene ID (NCBI) 64744
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ8WU79
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SMAP2 (Small Arf GAP Protein 2) is an Arf1 GTPase-activating protein. It localizes to early endosomes/TGN, binds clathrin/AP-1, and regulates retrograde transport from recycling endosomes to the Golgi. Critical for vesicle trafficking, organelle homeostasis, and spermatogenesis. (PMID: 26136365)

Protocols

Product Specific Protocols
WB protocol for SMAP2 antibody 88105-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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