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Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using SMAP2 Polyclonal antibody (16597-1-AP)

    WB analysis of human liver using 16597-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 16597-1-AP (SMAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse small intestine tissue using SMAP2 Polyclonal antibody (16597-1-AP)

    WB analysis of mouse small intestine using 16597-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 16597-1-AP (SMAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human colon tissue using SMAP2 Polyclonal antibody (16597-1-AP)

    WB analysis of human colon using 16597-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 16597-1-AP (SMAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using SMAP2 Polyclonal antibody (16597-1-AP)

    IHC staining of human liver using 16597-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16597-1-AP (SMAP2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using SMAP2 Polyclonal antibody (16597-1-AP)

    IHC staining of human liver using 16597-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16597-1-AP (SMAP2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SMAP2 Polyclonal antibody (16597-1-AP)

    IF Staining of HepG2 using 16597-1-AP

    Immunofluorescent analysis of HepG2 cells, using SMAP2 antibody 16597-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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SMAP2 Polyclonal antibody

SMAP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16597-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

RP1 228H13.3, small ArfGAP2, SMAP1L, SMAP2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman liver tissue, human colon tissue, mouse small intestine tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16597-1-AP targets SMAP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMAP2 fusion protein Ag9751 相同性解析による交差性が予測される生物種
Full Name small ArfGAP2
Calculated molecular weight 429 aa, 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC021133
Gene Symbol SMAP2
Gene ID (NCBI) 64744
RRIDAB_2066026
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WU79
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SMAP2 antibody 16597-1-APDownload protocol
IHC protocol for SMAP2 antibody 16597-1-APDownload protocol
IF protocol for SMAP2 antibody 16597-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols