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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMAD7 Monoclonal antibody (68594-1-Ig)

    WB analysis using 68594-1-Ig (same clone as 68594-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68594-1-Ig (SMAD7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68594-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SMAD7 Monoclonal antibody (68594-1-Ig)

    IF Staining of HeLa using 68594-1-Ig (same clone as 68594-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SMAD7 antibody (68594-1-Ig, Clone: 3D1C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68594-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U2OS cells using SMAD7 Monoclonal antibody (68594-1-Ig)

    FC experiment of U2OS using 68594-1-Ig (same clone as 68594-1-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug SMAD7 Monoclonal antibody (68594-1-Ig, Clone:3D1C3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68594-1-PBS in a different storage buffer formulation.

SMAD7 Monoclonal antibody, PBS Only

SMAD7 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 68594-1-PBS
Clone No.3D1C3

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

MADH7, MAD homolog 8, MAD homolog 7, hSMAD7, CRCS3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68594-1-PBS targets SMAD7 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag13688

Product name: Recombinant human SMAD7 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-426 aa of BC074819

Sequence: MFRTKRSALVRRLWRSRAPGGEDEEEGAGGGGGGGELRGEGATDSRAHGAGGGGPGRAGCCLGKAVRGAKGHHHPHPPAAGAGAAGGAEADLKALTHSVLKKLKERQLELLLQAVESRGGTRTACLLLPGRLDCRLGPGAPAGAQPAQPPSSYSLPLLLCKVFRWPDLRHSSEVKRLCCCESYGKINPELVCCNPHHLSRLCELESPPPPYSRYPMDFLKPTADCPDAVPSSAETGGTNYLAPGGLSDSQLLLEPGDRSHWCVVAYWEEKTRVGRLYCVQEPSLDIFYDLPQGNGFCLGQLNSDNKSQLVQKVRSKIGCGIQLTREVDGVWVYNRSSYPIFIKSATLDNPDSRTLLVHKVFPGFSIKAFDYEKAYSLQRPNDHEFMQQPWTGFTVQISFVKGWGQCYTRQFISSCPCWLEVIFNSR

相同性解析による交差性が予測される生物種
Full Name SMAD family member 7
Calculated molecular weight 426 aa, 46 kDa
Observed molecular weight45-55 kDa
GenBank accession numberBC074819
Gene Symbol SMAD7
Gene ID (NCBI) 4092
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO15105
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SMAD7, also named as Mothers against decapentaplegic homolog 7, is a 426 amino acid protein, which belongs to the dwarfin/SMAD family. SMAD7 Interaction with NEDD4L or RNF111 induces translocation from the nucleus to the cytoplasm (PubMed:16601693). TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta (PubMed:17327236). SMAD7 as antagonist of signaling by TGF-beta type 1 receptor superfamily members has been shown to inhibit TGF-beta and activin signaling by associating with their receptors thus preventing SMAD2 access. SMAD7 functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex and also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. SMAD7 positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

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