Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293T cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
18342-1-AP targets SMAD6 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SMAD6 fusion protein Ag13128 相同性解析による交差性が予測される生物種 |
| Full Name | SMAD family member 6 |
| Calculated molecular weight | 53 kDa |
| Observed molecular weight | 52 kDa |
| GenBank accession number | BC012986 |
| Gene Symbol | SMAD6 |
| Gene ID (NCBI) | 4091 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43541 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SMAD6 is an intracellular signal transduction protein that belongs to the SMAD protein family. It acts as a negative regulator in the transforming growth factor-beta (TGF-β) signaling pathway, binding to TGF-β receptors and inhibiting the phosphorylation of SMAD2/SMAD3, thereby blocking the transmission of TGF-β signals. Additionally, SMAD6 is involved in the regulation of the bone morphogenetic protein (BMP) signaling pathway, affecting cell proliferation, differentiation, and apoptosis. During development, SMAD6 is crucial for the formation of bones and cartilage. Its abnormal expression is associated with various diseases, including skeletal developmental abnormalities and certain types of cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SMAD6 antibody 18342-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Mol Med HNF1B inhibits cell proliferation via repression of SMAD6 expression in prostate cancer. |