Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 10231-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10231-1-AP (SMAD4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 10231-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10231-1-AP (SMAD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10231-1-AP
WB result of SMAD4 antibody (10231-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMAD4 transfected HEK-293 cells.
WB analysis using 10231-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10231-1-AP (SMAD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 10231-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10231-1-AP (SMAD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 10231-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 10231-1-AP (SMAD4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10231-1-AP
IP result of anti-SMAD4 (IP:10231-1-AP, 4ug; Detection:10231-1-AP 1:1500) with HeLa cells lysate 1320 ug.
IHC staining of human cervical cancer using 10231-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10231-1-AP (SMAD4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat small intestine using 10231-1-AP
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 10231-1-AP (SMAD4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 10231-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 10231-1-AP (SMAD4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse pancreas using 10231-1-AP
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10231-1-AP (SMAD4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse pancreas using 10231-1-AP
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10231-1-AP (SMAD4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat small intestine using 10231-1-AP
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 10231-1-AP (SMAD4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 10231-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10231-1-AP (SMAD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 10231-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10231-1-AP (SMAD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

SMAD4 Polyclonal antibody

SMAD4 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 10231-1-AP

Publications(139)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IHC, IF, IP, CoIP, ChIP, ELISA

Synonyms

DPC4, hSMAD4, JIP, MAD homolog 4, MADH4

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (139)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293T cells, COLO 320 cells, HCT 116 cells, HEK-293 cells, mouse liver tissue, NIH/3T3 cells, Neuro-2a cells, C6 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human skin cancer tissue, human tonsillitis tissue, human cervical cancer tissue, mouse pancreas tissue, rat small intestine tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 6 publications below
WB	See 111 publications below
IHC	See 17 publications below
IF	See 13 publications below
IP	See 6 publications below
CoIP	See 4 publications below
ChIP	See 9 publications below

Product Information

10231-1-AP targets SMAD4 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, bovine, hamster, sheep, goat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0299 Product name: Recombinant human SMAD4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 13-227 aa of BC002379 Sequence: NDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSNAPSSMMVKDEYVHDFEGQPSLSTEGHSIQTIQHPPSNRASTETYSTPALLAPSESNATSTANFPNIPVASTSQPAS 相同性解析による交差性が予測される生物種
Full Name	SMAD family member 4
Calculated molecular weight	60 kDa
Observed molecular weight	63-70 kDa
GenBank accession number	BC002379
Gene Symbol	SMAD4
Gene ID (NCBI)	4089
RRID	AB_2193323
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q13485
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Mammalian homologs of the Drosophila Mad gene include Smad1, Smad2, Smad3, Smad4 (DPC4), Smad5, Smad6, Smad7 and Smad8. Smad1 and Smad5 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities through interaction with various Smad family members . Smad6 and Smad7 regulate the response to activin/ TGF signaling by interfering with TGF -mediated phosphorylation of other Smad family members. This antibody is a rabbit polyclonal antibody raised against an internal region of human SMAD4.

Protocols

Product Specific Protocols
IHC protocol for SMAD4 antibody 10231-1-AP	Download protocol
IP protocol for SMAD4 antibody 10231-1-AP	Download protocol
WB protocol for SMAD4 antibody 10231-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IP,IF,ChIP	Signal Transduct Target Ther Annexin A1 binds PDZ and LIM domain 7 to inhibit adipogenesis and prevent obesity Authors - Lu Fang View Article
mouse	WB	Nature Post-translational control of beige fat biogenesis by PRDM16 stabilization Authors - Qiang Wang View Article
human	WB,IF	Mol Cancer HDAC6 orchestrates metastatic and immunosuppressive programs in small cell lung cancer through S100A2-TGF-β/SMAD and CSF1R signaling. Authors - Yantao Jiang View Article
mouse	WB	Cancer Res MPND Loss Epigenetically Activates TGF-β/SMAD3 Signaling to Drive Tumor Progression and Metastasis in Non-small Cell Lung Cancer. Authors - Jianfeng Yu View Article
mouse,human	WB,IHC,ChIP	Nat Commun Phosphorylation of FOXN3 by NEK6 promotes pulmonary fibrosis through Smad signaling Authors - Jinjin Yu View Article
		Nat Commun A Rras2-BMPR2 feedback loop sustains osteogenesis and represents a therapeutic target for osteoporosis. Authors - Renlei Yang View Article

Validation Data Gallery

WB analysis using 10231-1-AP

WB analysis using 10231-1-AP

WB analysis of HEK-293 using 10231-1-AP

WB analysis using 10231-1-AP

WB analysis of NIH/3T3 using 10231-1-AP

WB analysis of mouse liver using 10231-1-AP

IP experiment of HeLa using 10231-1-AP

IHC staining of human cervical cancer using 10231-1-AP

IHC staining of rat small intestine using 10231-1-AP

IHC staining of human skin cancer using 10231-1-AP

IHC staining of mouse pancreas using 10231-1-AP

IHC staining of mouse pancreas using 10231-1-AP

IHC staining of rat small intestine using 10231-1-AP

IHC staining of human tonsillitis using 10231-1-AP

IHC staining of human tonsillitis using 10231-1-AP

SMAD4 Polyclonal antibody

SMAD4 Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Annexin A1 binds PDZ and LIM domain 7 to inhibit adipogenesis and prevent obesity

Post-translational control of beige fat biogenesis by PRDM16 stabilization

HDAC6 orchestrates metastatic and immunosuppressive programs in small cell lung cancer through S100A2-TGF-β/SMAD and CSF1R signaling.

MPND Loss Epigenetically Activates TGF-β/SMAD3 Signaling to Drive Tumor Progression and Metastasis in Non-small Cell Lung Cancer.

Phosphorylation of FOXN3 by NEK6 promotes pulmonary fibrosis through Smad signaling

A Rras2-BMPR2 feedback loop sustains osteogenesis and represents a therapeutic target for osteoporosis.

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