Validation Data Gallery

Filter: AllWBIHC

WB analysis of Jurkat using 12050-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
WB analysis using 12050-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Y79 using 12050-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Y79 using 12050-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12050-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse colon using 12050-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 12050-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 12050-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12050-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of mouse colon using 12050-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 12050-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 12050-1-AP (SLU7 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of rat pancreas using 12050-1-AP
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 12050-1-AP (SLU7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat stomach using 12050-1-AP
Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 12050-1-AP (SLU7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse pancreas using 12050-1-AP
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 12050-1-AP (SLU7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse pancreas using 12050-1-AP
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 12050-1-AP (SLU7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

SLU7 Polyclonal antibody

SLU7 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12050-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, RIP, IHC, ELISA

Synonyms

9G8, hSlu7, Pre mRNA splicing factor SLU7, SLU7

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Jurkat cells, HeLa cells, mouse colon tissue, K-562 cells, Y79 cells
Positive IHC detected in	mouse pancreas tissue, human colon cancer tissue, rat pancreas tissue, rat stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 1 publications below
IHC	See 1 publications below
IF	See 1 publications below
RIP	See 1 publications below

Product Information

12050-1-AP targets SLU7 in WB, IF, RIP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SLU7 fusion protein Ag2680 相同性解析による交差性が予測される生物種
Full Name	SLU7 splicing factor homolog (S. cerevisiae)
Calculated molecular weight	586 aa, 68 kDa
Observed molecular weight	68 kDa
GenBank accession number	BC010634
Gene Symbol	SLU7
Gene ID (NCBI)	10569
RRID	AB_10663102
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O95391
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Pre-mRNA splicing occurs in two sequential transesterification steps. SLU7 is a splicing factor that has been found to be essential during the second catalytic step in the pre-mRNA splicing process. It associates with the spliceosome and contains a zinc knuckle motif that is found in other splicing factors and is involved in protein-nucleic acid and protein-protein interactions.

Protocols

Product Specific Protocols
WB protocol for SLU7 antibody 12050-1-AP	Download protocol
IHC protocol for SLU7 antibody 12050-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	RIP	Mol Cancer A novel polypeptide CAPG-171aa encoded by circCAPG plays a critical role in triple-negative breast cancer Authors - Runjie Song View Article
human,mouse	WB,IHC,IF	Adv Sci (Weinh) G3BP1 and SLU7 Jointly Promote Immune Evasion by Downregulating MHC-I via PI3K/Akt Activation in Bladder Cancer Authors - Xianchong Zheng KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of Jurkat using 12050-1-AP

WB analysis using 12050-1-AP

WB analysis of Y79 using 12050-1-AP

WB analysis of Y79 using 12050-1-AP

WB analysis of HeLa using 12050-1-AP

WB analysis of mouse colon using 12050-1-AP

WB analysis of K-562 using 12050-1-AP