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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using SLTM Polyclonal antibody (17889-1-AP)

    WB analysis of mouse brain using 17889-1-AP (same clone as 17889-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17889-1-AP (SLTM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17889-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SLTM Polyclonal antibody (17889-1-AP)

    WB analysis of HeLa using 17889-1-AP (same clone as 17889-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 17889-1-AP (SLTM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17889-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SLTM Polyclonal antibody (17889-1-AP)

    WB analysis of HeLa using 17889-1-AP (same clone as 17889-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 17889-1-AP (SLTM antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17889-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using SLTM Polyclonal antibody (17889-1-AP)

    IHC staining of human skin cancer using 17889-1-AP (same clone as 17889-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 17889-1-AP (SLTM antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17889-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SLTM Polyclonal antibody (17889-1-AP)

    IF Staining of HepG2 using 17889-1-AP (same clone as 17889-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SLTM antibody (17889-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 17889-1-PBS in a different storage buffer formulation.

SLTM Polyclonal antibody, PBS Only

SLTM Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 17889-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

SAFB-like transcription modulator, Modulator of estrogen-induced transcription, MET

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17889-1-PBS targets SLTM in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12060

Product name: Recombinant human SLTM protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 154-502 aa of BC046119

Sequence: RERIRIEQERRKEAERIAREREELRRQQQQLRYEQEKRNSLKRPRDVDHRRDDPYWSENKKLSLDTDARFGHGSDYSRQQNRFNDFDHRERGRFPESSAVQSSSFERRDRFVGQSEGKKARPTARREDPSFERYPKNFSDSRRNEPPPPRNELRESDRREVRGERDERRTVIIHDRPDITHPRHPREAGPNPSRPTSWKSEGSMSTDKRETRVERPERSGREVSGHSVRGAPPGNRSSASGYGSREGDRGVITDRGGGSQHYPEERHVVERHGRDTSGPRKEWHGPPSQGPSYHDTRRMGDGRAGAGMITQHSSNASPINRIVQISGNSMPRGSGSGFKPFKGGPPRRF

相同性解析による交差性が予測される生物種
Full Name SAFB-like, transcription modulator
Calculated molecular weight478aa,57 kDa; 1034aa,117 kDa
Observed molecular weight 135-140 kDa
GenBank accession numberBC046119
Gene Symbol SLTM
Gene ID (NCBI) 79811
RRIDAB_2192729
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NWH9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SLTM, also named as Modulator of estrogen-induced transcription, is a 1034 amino acid protein, which localizes in the nucleus. When it is overexpressed, SLTM acts as a general inhibitor of transcription that eventually leads to apoptosis. The calculated molecular weight of SLTM is 117 kDa, but modified SLTM is about 135-140 kDa.

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