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Validation Data Gallery

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  • Western Blot (WB) analysis of human saliva using SLPI Recombinant antibody (83965-4-RR)

    WB analysis of human saliva using 83965-4-RR (same clone as 83965-4-PBS)

    human saliva were subjected to SDS PAGE followed by western blot with 83965-4-RR (SLPI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83965-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using SLPI Recombinant antibody (83965-4-RR)

    WB analysis of PC-3 using 83965-4-RR (same clone as 83965-4-PBS)

    WB result of SLPI antibody (83965-4-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLPI transfected PC-3 cells. This data was developed using the same antibody clone with 83965-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using SLPI Recombinant antibody (83965-4-RR)

    IHC staining of human cervical cancer using 83965-4-RR (same clone as 83965-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 83965-4-RR (SLPI antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83965-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using SLPI Recombinant antibody (83965-4-RR)

    IHC staining of human cervical cancer using 83965-4-RR (same clone as 83965-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 83965-4-RR (SLPI antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83965-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83965-4-RR

    Affinity and Kinetic Characterization of 83965-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83965-4-RR against Human SLPI were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

SLPI Recombinant monoclonal antibody, PBS Only

SLPI Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 83965-4-PBS
Clone No.241057F8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

241057F8, ALK1, ALP, Antileukoprotease, Antileukoproteinase

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83965-4-PBS targets SLPI in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag1673

Product name: Recombinant human SLPI protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-132 aa of BC020708

Sequence: MKSSGLFPFLVLLALGTLAPWAVEGSGKSFKAGVCPPKKSAQCLRYKKPECQSDWQCPGKKRCCPDTCGIKCLDPVDTPNPTRRKPGKCPVTYGQCLMLNPPNFCEMDGQCKRDLKCCMGMCGKSCVSPVKA

相同性解析による交差性が予測される生物種
Full Name secretory leukocyte peptidase inhibitor
Calculated molecular weight 14 kDa
Observed molecular weight14 kDa
GenBank accession numberBC020708
Gene Symbol SLPI
Gene ID (NCBI) 6590
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP03973
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SLPI, a member of Whey acidic protein family, could mediate a variety of biological processes including cell proliferation and apoptosis, repairing reaction and immune response, with crucial roles in anti-protease, anti-inflammatory, anti-bacterial and anti-virus. SLPI is mainly expressed in epithelial cells.

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