Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse heart tissue, A431 cells, mouse skeletal muscle tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
Product Information
25220-1-AP targets SLMAP in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18162 Product name: Recombinant human SLMAP protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 479-828 aa of BC115701 Sequence: VSLLKDDLQGAQSEIEAKQEIQHLRKELIEAQELARTSKQKCFELQALLEEERKAYRNQVEESTKQIQVLQAQLQRLHIDTENLREEKDSEITSTRDELLSARDEILLLHQAAAKVASERDTDIASLQEELKKVRAELERWRKAASEYEKEITSLQNSFQLRCQQCEDQQREEATRLQGELEKLRKEWNALETECHSLKRENVLLSSELQRQEKELHNSQKQSLELTSDLSILQMSRKELENQVGSLKEQHLRDSADLKTLLSKAENQAKDVQKEYEKTQTVLSELKLKFEMTEQEKQSITDELKQCKNNLKLLREKGNNKPWPWMPMLAALVAVTAIVLYVPGLARASP 相同性解析による交差性が予測される生物種 |
| Full Name | sarcolemma associated protein |
| Calculated molecular weight | 828 aa, 95 kDa |
| Observed molecular weight | 35 kDa, 45 kDa, 63-66 kDa, 83-91 kDa |
| GenBank accession number | BC115701 |
| Gene Symbol | SLMAP |
| Gene ID (NCBI) | 7871 |
| RRID | AB_2879968 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14BN4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Sarcolemmal membrane associated protein (SLMAP) is a tail-anchored membrane protein involved in the docking and membrane fusion process via protein-protein interactions. Deregulation of SLMAP expression plays an important role in type 2 diabetes, and changes in SLMAP expression are linked to hyperglycemia and diabetes. SLMAPs are components of cardiac membranes and have been identified as a novel regulator of cardiac function. There are several isoforms of SLMAPs with varying molecular size (35, 45, 63, and 83-91 kDa) as a result of alternative splicing. The short 35-kDa isoform is predominantly expressed in cardiac and slow-twitch skeletal muscle, while the 83- to 91-kDa isoform is ubiquitous. This antibody recognizes all of these isoforms in mouse heart lysate. (21785580, 22180652)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SLMAP antibody 25220-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |