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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLK Polyclonal antibody (19743-1-AP)

    WB analysis using 19743-1-AP (same clone as 19743-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19743-1-AP (SLK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19743-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using SLK Polyclonal antibody (19743-1-AP)

    WB analysis of human brain using 19743-1-AP (same clone as 19743-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 19743-1-AP (SLK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19743-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using SLK Polyclonal antibody (19743-1-AP)

    WB analysis of MCF-7 using 19743-1-AP (same clone as 19743-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 19743-1-AP (SLK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19743-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using SLK Polyclonal antibody (19743-1-AP)

    WB analysis of mouse liver using 19743-1-AP (same clone as 19743-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 19743-1-AP (SLK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19743-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SLK Polyclonal antibody (19743-1-AP)

    IF Staining of HepG2 using 19743-1-AP (same clone as 19743-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SLK antibody (19743-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 19743-1-PBS in a different storage buffer formulation.

SLK Polyclonal antibody, PBS Only

SLK Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 19743-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19743-1-PBS targets SLK in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name STE20-like kinase (yeast)
Calculated molecular weight 143 kDa
Observed molecular weight 143-160 kDa, 210-220 kDa
GenBank accession numberNM_014720
Gene Symbol SLK
Gene ID (NCBI) 9748
RRIDAB_10644278
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H2G2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SLK, also named as KIAA0204 and STK2, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. SLK plays an important role in cell proliferation and cytoskeletal remodeling. In fibroblasts, SLK has been shown to respond to FAK/Src signaling and regulate focal adhesion turnover through Paxillin phosphorylation. Full-length SLK (~220 kDa) has also been shown to be essential for embryonic development. In myoblasts, the overexpression of a dominant negative SLK is sufficient to block myoblast fusion. (PMID: 28153048, PMID: 25882817, PMID: 23815977)

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