Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 26006-1-AP (same clone as 26006-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 26006-1-AP (SLIRP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26006-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 26006-1-AP (same clone as 26006-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 26006-1-AP (SLIRP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26006-1-PBS in a different storage buffer formulation.
WB analysis of PC-3 using 26006-1-AP (same clone as 26006-1-PBS)
PC-3 cells were subjected to SDS PAGE followed by western blot with 26006-1-AP (SLIRP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26006-1-PBS in a different storage buffer formulation.
WB analysis of MDA-MB-453s using 26006-1-AP (same clone as 26006-1-PBS)
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 26006-1-AP (SLIRP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26006-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 26006-1-AP (same clone as 26006-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26006-1-AP (SLIRP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26006-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 26006-1-AP (same clone as 26006-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26006-1-AP (SLIRP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26006-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 26006-1-AP (same clone as 26006-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26006-1-AP (SLIRP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26006-1-PBS in a different storage buffer formulation.
IHC staining of human ovary cancer using 26006-1-AP (same clone as 26006-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 26006-1-AP (SLIRP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26006-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 26006-1-AP (same clone as 26006-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26006-1-AP (SLIRP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26006-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 26006-1-AP (same clone as 26006-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26006-1-AP (SLIRP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26006-1-PBS in a different storage buffer formulation.
IF Staining of PC-3 using 26006-1-AP (same clone as 26006-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using SLIRP antibody (26006-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26006-1-PBS in a different storage buffer formulation.
IF Staining of PC-3 using 26006-1-AP (same clone as 26006-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using SLIRP antibody (26006-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26006-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

SLIRP Polyclonal antibody, PBS Only

SLIRP Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 26006-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

C14orf156, DC23, DC50, PD04872, SRA stem-loop-interacting RNA-binding protein, mitochondrial

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

26006-1-PBS targets SLIRP in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag22945 Product name: Recombinant human C14orf156 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-109 aa of BC017895 Sequence: MAASAARGAAALRRSINQPVAFVRRIPWTAASSQLKEHFAQFGHVRRCILPFDKETGFHRGLGWVQFSSEEGLRNALQQENHIIDGVKVQVHTRRPKLPQTSDDEKKDF 相同性解析による交差性が予測される生物種
Full Name	chromosome 14 open reading frame 156
Calculated molecular weight	109 aa, 12 kDa
Observed molecular weight	12 kDa
GenBank accession number	BC017895
Gene Symbol	SLIRP
Gene ID (NCBI)	81892
RRID	AB_2880332
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9GZT3
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

SLIRP, also named as C14orf156, DC23, DC50 and PD04872. It is an RNA-binding protein on steroid receptor RNA activator (SRA), and its expression is ubiquitous in human normal tissues. SLIRP can affect mitochondrial mRNA transcription and energy conversion. In addition, the loss or weakening of SLIRP destroys part of the structure of mitochondria, affects mitochondria energy metabolism, and increases sperm or cell oxidative stress damage (PMID: 33150185). SLIRP has 2 isoforms with the molecular mass of 12 kDa.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 26006-1-AP (same clone as 26006-1-PBS)

WB analysis of HeLa using 26006-1-AP (same clone as 26006-1-PBS)

WB analysis of PC-3 using 26006-1-AP (same clone as 26006-1-PBS)

WB analysis of MDA-MB-453s using 26006-1-AP (same clone as 26006-1-PBS)

IHC staining of human kidney using 26006-1-AP (same clone as 26006-1-PBS)

IHC staining of mouse testis using 26006-1-AP (same clone as 26006-1-PBS)

IHC staining of human stomach cancer using 26006-1-AP (same clone as 26006-1-PBS)

IHC staining of human ovary cancer using 26006-1-AP (same clone as 26006-1-PBS)