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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse thymus tissue using SLD5 Polyclonal antibody (13315-1-AP)

    WB analysis of mouse thymus using 13315-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13315-1-AP (SLD5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using SLD5 Polyclonal antibody (13315-1-AP)

    WB analysis of mouse testis using 13315-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13315-1-AP (SLD5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SLD5 Polyclonal antibody (13315-1-AP)

    IF Staining of HeLa using 13315-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SLD5 antibody (13315-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

SLD5 Polyclonal antibody

SLD5 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 13315-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

GINS4, GINS complex subunit 4, DNA replication complex GINS protein SLD5, N-terminally processed

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse thymus tissue, mouse testis tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13315-1-AP targets SLD5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLD5 fusion protein Ag4158 相同性解析による交差性が予測される生物種
Full Name GINS complex subunit 4 (Sld5 homolog)
Calculated molecular weight 223 aa, 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC027454
Gene Symbol SLD5
Gene ID (NCBI) 84296
RRIDAB_2109671
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BRT9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLD5 (DNA replication complex GINS protein SLD5) is also named as GINS4 (GINS complex subunit 4). SLD5 is required for correct functioning of the GINS complex, a complex that plays an essential role in the initiation of DNA replication, and progression of DNA replication forks (PMID: 17417653). SLD5 forms a GINS complex with PSF1, PSF2 and PSF3, which is essential for the initiation of DNA replication in lower eukaryotes (PMID: 24244394). SLD5 is a member of the GINS complex, essential for DNA replication in eukaryotes. It has been reported that SLD5 is involved in early embryogenesis in the mouse, and cell cycle progression and genome integrity in Drosophila (PMID: 27499248).

Protocols

Product Specific Protocols
WB protocol for SLD5 antibody 13315-1-APDownload protocol
IF protocol for SLD5 antibody 13315-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols