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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLCO2B1 Polyclonal antibody (55180-1-AP)

    WB analysis using 55180-1-AP (same clone as 55180-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55180-1-AP (SLCO2B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55180-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SLCO2B1 Polyclonal antibody (55180-1-AP)

    WB analysis using 55180-1-AP (same clone as 55180-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55180-1-AP (SLCO2B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55180-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using SLCO2B1 Polyclonal antibody (55180-1-AP)

    IHC staining of human liver using 55180-1-AP (same clone as 55180-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55180-1-AP (SLCO2B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55180-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SLCO2B1 Polyclonal antibody (55180-1-AP)

    IF Staining of HepG2 using 55180-1-AP (same clone as 55180-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SLCO2B1 antibody (55180-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 55180-1-PBS in a different storage buffer formulation.

SLCO2B1 Polyclonal antibody, PBS Only

SLCO2B1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 55180-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

SLC21A9, OATPRP2, OATPB, OATP2B1, OATP B

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55180-1-PBS targets SLCO2B1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name solute carrier organic anion transporter family, member 2B1
Calculated molecular weight 77 kDa
Observed molecular weight61~74 kDa
GenBank accession numberNM_007256
Gene Symbol SLCO2B1
Gene ID (NCBI) 11309
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO94956
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Solute carrier organic anion (SLCO) gene families encode organic anion transport proteins (OATP), which are membrane transporters widely expressed in the human body that influx numerous compounds and drugs including androgens. SLCO2B1 is a member of an organic anion transporter family and has previously been shown to transport a multitude of drugs and steroid hormone conjugates (PMID: 35714613). High expression of SLCO2B1 is known to associate with the resistance to androgen deprivation therapy in prostate cancer (PMID: 29581838).

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