Validation Data Gallery
Tested Applications
| Positive WB detected in | unboiled mouse brain tissue, boiled mouse brain tissue |
| Positive IP detected in | rat brain tissue, mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13877-1-AP targets SLC9A6 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4958 Product name: Recombinant human SLC9A6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 500-701 aa of BC049169 Sequence: RDTATYARQMMFSTTLLIVFFTVWVFGGGTTAMLSCLHIRVGVDSDQEHLGVPENERRTTKAESAWLFRMWYNFDHNYLKPLLTHSGPPLTTTLPACCGPIARCLTSPQAYENQEQLKDDDSDLILNDGDISLTYGDSTVNTEPATSSAPRRFMGNSSEDALDRELAFGDHELVIRGTRLVLPMDDSEPPLNLLDNTRHGPA 相同性解析による交差性が予測される生物種 |
| Full Name | solute carrier family 9 (sodium/hydrogen exchanger), member 6 |
| Calculated molecular weight | 74 kDa |
| Observed molecular weight | 72-78 kDa |
| GenBank accession number | BC049169 |
| Gene Symbol | SLC9A6 |
| Gene ID (NCBI) | 10479 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92581 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The SLC9A6 gene, also known as NHE6 gene, is located on chromosome Xq26.3 and encodes a monovalent sodium-selective sodium/hydrogen exchanger (NHE) (PMID: 27142213). Loss-of-function mutations in endosomal Na+/H+ exchanger 6 (NHE6) encoded by SLC9A6 cause the X-linked neurologic disorder CS (PMID: 18342287). Slc9a6 loss of function causes the relevant defects, including disrupted endosomal-lysosomal function, neurodevelopmental and neurodegenerative pathology, impaired plasticity and impoverished neuronal arborization (PMID: 36621975). The SLC9A6 protein is mainly expressed in human brain tissues (PMID: 9039502). It is found in the membranes of intracellular organelles such as mitochondria and endosomes, playing an essential role in regulating endosomal pH and volume (PMID: 40722028).
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for SLC9A6 antibody 13877-1-AP | Download protocol |
| WB protocol for SLC9A6 antibody 13877-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |