Validation Data Gallery
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Tested Applications
| Positive WB detected in | T-47D cells, MCF-7 cells, HeLa cells, HEK-293T cells, U-251 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84060-5-RR targets SLC9A3R2 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | SLC9A3R2 fusion protein Ag13476 相同性解析による交差性が予測される生物種 |
| Full Name | solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 2 |
| Calculated molecular weight | 337 aa, 37 kDa |
| Observed molecular weight | ~45 kDa |
| GenBank accession number | BC106001 |
| Gene Symbol | SLC9A3R2 |
| Gene ID (NCBI) | 9351 |
| RRID | AB_3671630 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q15599 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SLC9A3R2, also named as NHERF2, is a member of the Na+/H+ exchanger regulatory factor (NHERF) family of PDZ scaffolding proteins. SLC9A3R2 is needed for cGMP and carbachol-mediated inhibition as well as LPA and dexamethasone-mediated stimulation of NHE3. NHERF1 and NHERF2 are highly homologous structurally to each other but play different roles in the regulation of Na+ and Cl− transport.(PMID: 23612977)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC9A3R2 antibody 84060-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |