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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC6A9 / GlyT1 Polyclonal antibody (30971-1-AP)

    WB analysis using 30971-1-AP

    BxPC-3 cells lysates were subjected to SDS PAGE followed by western blot with 30971-1-AP (SLC6A9 / GlyT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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SLC6A9 / GlyT1 Polyclonal antibody

SLC6A9 / GlyT1 Polyclonal Antibody for WB, ELISA
Cat No. 30971-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inBxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30971-1-AP targets SLC6A9 / GlyT1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC6A9 / GlyT1 fusion protein Ag34437 相同性解析による交差性が予測される生物種
Full Name solute carrier family 6 (neurotransmitter transporter, glycine), member 9
Calculated molecular weight706 aa, 78 kDa
Observed molecular weight60 kDa
GenBank accession numberNM_201649
Gene Symbol SLC6A9
Gene ID (NCBI) 6536
RRIDAB_3669799
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP48067
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC6A9, also named glycine transporter 1 (GlyT1), is a transporter protein of the SLC6 family of sodium- and chloride-dependent neurotransmitter transporters. GlyT1 is the main regulator of synaptic glycine concentrations, assisted by the neuronal GlyT2 (PMID: 28828604). The 55-60 kDa band is the partially glycosylated form of GlyT1 (PMID: 27874011, PMID: 8995423).

Protocols

Product Specific Protocols
WB protocol for SLC6A9 / GlyT1 antibody 30971-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols