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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC6A8 Polyclonal antibody (20299-1-AP)

    WB analysis using 20299-1-AP (same clone as 20299-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20299-1-AP (SLC6A8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20299-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HUVEC cells using SLC6A8 Polyclonal antibody (20299-1-AP)

    WB analysis of HUVEC using 20299-1-AP (same clone as 20299-1-PBS)

    HUVEC cells were subjected to SDS PAGE followed by western blot with 20299-1-AP (SLC6A8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20299-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SLC6A8 Polyclonal antibody (20299-1-AP)

    IHC staining of mouse brain using 20299-1-AP (same clone as 20299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20299-1-AP (SLC6A8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20299-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SLC6A8 Polyclonal antibody (20299-1-AP)

    IHC staining of mouse brain using 20299-1-AP (same clone as 20299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20299-1-AP (SLC6A8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20299-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SLC6A8 Polyclonal antibody (20299-1-AP)

    IHC staining of mouse brain using 20299-1-AP (same clone as 20299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20299-1-AP (SLC6A8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20299-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SLC6A8 Polyclonal antibody (20299-1-AP)

    IHC staining of mouse brain using 20299-1-AP (same clone as 20299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20299-1-AP (SLC6A8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20299-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SLC6A8 Polyclonal antibody (20299-1-AP)

    IF Staining of mouse brain using 20299-1-AP (same clone as 20299-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 20299-1-AP (SLC6A8 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20299-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

SLC6A8 Polyclonal antibody, PBS Only

SLC6A8 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 20299-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Sodium- and chloride-dependent creatine transporter 1, CT1, CRTR, CRT, Creatine transporter 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20299-1-PBS targets SLC6A8 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC6A8 fusion protein Ag14110 相同性解析による交差性が予測される生物種
Full Name solute carrier family 6 (neurotransmitter transporter, creatine), member 8
Calculated molecular weight 635 aa, 71 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC012355
Gene Symbol SLC6A8
Gene ID (NCBI) 6535
RRIDAB_2878665
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP48029
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SLC6A8, also known as the sodium- and chloride-dependent creatine transporter 1 (CT1), plays a critical role in transporting creatine, a crucial molecule for energy metabolism, into cells. SLC6A8 belongs to the solute carrier family 6 (SLC6), responsible for transporting diverse molecules across cell membranes. SLC6A8 expression is highest in muscle, kidney, and other tissues with high energy demands. Mutations in SLC6A8 cause creatine transporter deficiency, an X-linked mental retardation disorder (PMID: 17465020).