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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse brain tissue using SLC6A7 Recombinant antibody (83412-4-RR)

    IHC staining of mouse brain using 83412-4-RR (same clone as 83412-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83412-4-RR (SLC6A7 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83412-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SLC6A7 Recombinant antibody (83412-4-RR)

    IHC staining of mouse brain using 83412-4-RR (same clone as 83412-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83412-4-RR (SLC6A7 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83412-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using SLC6A7 Recombinant antibody (83412-4-RR)

    IHC staining of rat brain using 83412-4-RR (same clone as 83412-4-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83412-4-RR (SLC6A7 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83412-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using SLC6A7 Recombinant antibody (83412-4-RR)

    IHC staining of rat brain using 83412-4-RR (same clone as 83412-4-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83412-4-RR (SLC6A7 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83412-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83412-4-RR

    Affinity and Kinetic Characterization of 83412-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83412-4-RR against Human SLC6A7 were performed. The affinity constant is below 1 pM.

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SLC6A7 Recombinant antibody, PBS Only

SLC6A7 Uni-rAbTM Recombinant Antibody for IHC, ELISA
Cat No. 83412-4-PBS
Clone No.240395H5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Synonyms

Sodium-dependent proline transporter, PROT, 240395H5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83412-4-PBS targets SLC6A7 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SLC6A7 fusion protein Ag34413 相同性解析による交差性が予測される生物種
Full Name solute carrier family 6 (neurotransmitter transporter, L-proline), member 7
Calculated molecular weight 636 aa, 71 kDa
GenBank accession numberBC093785
Gene Symbol SLC6A7
Gene ID (NCBI) 6534
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ99884
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SLC6A7 also known as PROT, belongs to the solute Carrier 6 gene family. SLC6A7 is a high-affinity mammalian brain L-proline transporter, which is different from other sodium-dependent plasma membrane carriers, and has unique pharmacological specificity, kinetic properties, and ionic requirements. SLC6A7 is a brain specific sodium (and chloride)-dependent proline transporter, that terminates the action of proline by its high affinity sodium-dependent reuptake into presynaptic terminals(PMID: 7651355).