Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse eye tissue |
| Positive IHC detected in | rat eye tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
26725-1-AP targets SLC6A6/TAUT in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24935 Product name: Recombinant human SLC6A6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 564-620 aa of BC137128 Sequence: RLCQTEGPFLVRVKYLLTPREPNRWAVEREGATPYNSRTVMNGALVKPTHIIVETMM 相同性解析による交差性が予測される生物種 |
| Full Name | solute carrier family 6 (neurotransmitter transporter, taurine), member 6 |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC137128 |
| Gene Symbol | SLC6A6/TAUT |
| Gene ID (NCBI) | 6533 |
| RRID | AB_3085896 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P31641 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SLC6A6, also known as TAUT, belongs to the solute carrier 6 (SLC6) family. SLC6A6 is expressed in retinal capillary endothelial cells and mediates the transport of taurine. SLC6A6 can also mediate the transport of beta-alanine, hypotaurine, and gamma-aminobutyric acid. SLC6A6 has 2 isoforms with the molecular mass of 23 and 70 kDa. Sometimes bands of 50 kDa (non-glycosylated) and 75 kDa (glycosylated) may be detected refer to literature (PMID: 35050188).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SLC6A6/TAUT antibody 26725-1-AP | Download protocol |
| WB protocol for SLC6A6/TAUT antibody 26725-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Invest Ophthalmol Vis Sci SLC6A6-Mediated Taurine Uptake Sustains Corneal Epithelial Stem/Progenitor Cell Function to Counteract Age-Related Dysfunction | ||
Placenta Gestational TCBPA exposure impairs placental function and fetal Development: Mechanisms of syncytialization disruption and fetal growth restriction |