Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, mouse kidney tissue, rat kidney tissue |
| Positive IHC detected in | rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29827-1-AP targets SLC5A8 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30183 Product name: Recombinant human SLC5A8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 461-518 aa of BC110492 Sequence: QIYPPLPERTLPLHLDIQGCNSTYNETNLMTTTEMPFTTSVFQIYNVQRTPLMDNWYS 相同性解析による交差性が予測される生物種 |
| Full Name | solute carrier family 5 (iodide transporter), member 8 |
| Calculated molecular weight | 610 aa, 67 kDa |
| Observed molecular weight | 67 kDa |
| GenBank accession number | BC110492 |
| Gene Symbol | SLC5A8 |
| Gene ID (NCBI) | 160728 |
| RRID | AB_3086176 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N695 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The sodium-coupled monocarboxylate transporter 1 (SMCT1; SLC5A8), belonging to the SLC5A family, is a 67-kDa protein that functions as a Na+-coupled electrogenic transporter for SCFAs. SLC5A mRNA and protein are expressed in healthy human, rat, and mouse colon. The protein is localized to the apical membrane of the colonocytes. Immunohistochemical studies revealed the selective localization of SMCT1 on the brush border in the villi of the terminal ileum and crypts of the colon in mice.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SLC5A8 antibody 29827-1-AP | Download protocol |
| WB protocol for SLC5A8 antibody 29827-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |