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Validation Data Gallery

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  • Western Blot (WB) analysis of Caco-2 cells using SLC5A6 Polyclonal antibody (26407-1-AP)

    WB analysis of Caco-2 using 26407-1-AP

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 26407-1-AP (SLC5A6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using SLC5A6 Polyclonal antibody (26407-1-AP)

    WB analysis of mouse brain using 26407-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 26407-1-AP (SLC5A6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse small intestine tissue using SLC5A6 Polyclonal antibody (26407-1-AP)

    WB analysis of mouse small intestine using 26407-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 26407-1-AP (SLC5A6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using SLC5A6 Polyclonal antibody (26407-1-AP)

    WB analysis of COLO 320 using 26407-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 26407-1-AP (SLC5A6 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using SLC5A6 Polyclonal antibody (26407-1-AP)

    WB analysis of rat brain using 26407-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 26407-1-AP (SLC5A6 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using SLC5A6 Polyclonal antibody (26407-1-AP)

    WB analysis of COLO 320 using 26407-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 26407-1-AP (SLC5A6 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using SLC5A6 Polyclonal antibody (26407-1-AP)

    IP experiment of mouse brain using 26407-1-AP

    IP result of anti-SLC5A6 (IP:26407-1-AP, 4ug; Detection:26407-1-AP 1:500) with mouse brain tissue lysate 5200ug.

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SLC5A6 Polyclonal antibody

SLC5A6 Polyclonal Antibody for WB, IP, ELISA
Cat No. 26407-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

Na(+)-dependent multivitamin transporter, SMVT, Sodium-dependent multivitamin transporter

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inCaco-2 cells, mouse brain tissue, mouse small intestine tissue, COLO 320 cells, rat brain tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

26407-1-AP targets SLC5A6 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC5A6 fusion protein Ag23907 相同性解析による交差性が予測される生物種
Full Name solute carrier family 5 (sodium-dependent vitamin transporter), member 6
Observed molecular weight 69 kDa
GenBank accession numberBC012806
Gene Symbol SLC5A6
Gene ID (NCBI) 8884
RRIDAB_2880502
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y289
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC5A6, also known as the sodium-dependent multivitamin transporter (hSMVT), is a transmembrane protein that plays a crucial role in the uptake of essential vitamins, including biotin, pantothenic acid, and lipoic acid. This protein is widely expressed in various human tissues, including the placenta, intestine, brain, liver, lung, kidney, cornea, retina, and heart. It uses energy from the transmembrane sodium ion gradient and membrane potential to actively transport these vitamins into cells. Due to different glycosylation modifications, SLC5A6 may exhibit two bands at ~55 kDa and ~69 kDa. (PMID: 20980265, PMID: 25971966)

Protocols

Product Specific Protocols
WB protocol for SLC5A6 antibody 26407-1-APDownload protocol
IP protocol for SLC5A6 antibody 26407-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanWB

Nat Metab

Vitamin B5 supports MYC oncogenic metabolism and tumor progression in breast cancer

Authors - Peter Kreuzaler
View Article
humanWB

Mol Cell Proteomics

Hypoxia is a dominant remodeler of the effector T cell surface proteome relative to activation and regulatory T cell suppression.

Authors - James R Byrnes
View Article