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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC5A11 Polyclonal antibody (14089-1-AP)

    WB analysis using 14089-1-AP

    C2C12 cells were subjected to SDS PAGE followed by western blot with 14089-1-AP (SLC5A11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SLC5A11 Polyclonal antibody (14089-1-AP)

    IHC staining of human colon cancer using 14089-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14089-1-AP (SLC5A11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SLC5A11 Polyclonal antibody (14089-1-AP)

    IHC staining of human colon cancer using 14089-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14089-1-AP (SLC5A11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using SLC5A11 Polyclonal antibody (14089-1-AP)

    IHC staining of mouse skeletal muscle using 14089-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14089-1-AP (SLC5A11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using SLC5A11 Polyclonal antibody (14089-1-AP)

    IHC staining of mouse skeletal muscle using 14089-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14089-1-AP (SLC5A11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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SLC5A11 Polyclonal antibody

SLC5A11 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14089-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

KST1, Na(+)/myo-inositol cotransporter 2, RKST1, SGLT6, SLGTX

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inC2C12 cells
Positive IHC detected inhuman colon cancer tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

14089-1-AP targets SLC5A11 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC5A11 fusion protein Ag5220 相同性解析による交差性が予測される生物種
Full Name solute carrier family 5 (sodium/glucose cotransporter), member 11
Calculated molecular weight 611 aa, 67 kDa
Observed molecular weight 74 kDa
GenBank accession numberBC057780
Gene Symbol SLC5A11
Gene ID (NCBI) 115584
RRIDAB_2189540
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WWX8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC5A11 (Sodium/myo-inositol cotransporter 2 ), also known as KST1, SMIT2, is a sodium/solute cotransporter-like neuronal protein. In Drosophila, SLC5A11 mediates hunger by regulating potassium channel activity. SLC5A11 has been linked to infantile convulsions and paroxysmal dyskinesia (ICCA syndrome) and benign familial infantile convulsions (BFIC) (PMID: 27397890).

Protocols

Product Specific Protocols
WB protocol for SLC5A11 antibody 14089-1-APDownload protocol
IHC protocol for SLC5A11 antibody 14089-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Clin Endocrinol Metab

Placental Inositol Reduced in Gestational Diabetes as Glucose Alters Inositol Transporters and IMPA1 Enzyme Expression.

Authors - Reshma A Pillai
View Article
humanWB

Front Pharmacol

Quercetin Attenuates Atherosclerosis via Modulating Oxidized LDL-Induced Endothelial Cellular Senescence.

Authors - Yue-Hua Jiang
View Article