Validation Data Gallery
Tested Applications
| Positive IHC detected in | human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30396-1-AP targets SLC52A2 in IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag33252 Product name: Recombinant human GPR172A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 217-276 aa of BC002917 Sequence: LLPPPPSVPTGELGSGLQVGAPGAEEEVEESSPLQEPPSQAAGTTPGPDPKAYQLLSARS 相同性解析による交差性が予測される生物種 |
| Full Name | Solute carrier family 52, riboflavin transporter, member 2 |
| Calculated molecular weight | 46 kDa |
| GenBank accession number | BC002917 |
| Gene Symbol | SLC52A2 |
| Gene ID (NCBI) | 79581 |
| RRID | AB_3742338 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HAB3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SLC52A2 (Also known as RFVT2, RFT3) is a membrane protein that belongs to the riboflavin transporter family. SLC52A2 is a transmembrane protein that mediates cellular uptake of riboflavin. Humans are unable to synthesize vitamin B2/riboflavin and must obtain it via intestinal absorption (PubMed:20463145). Mutations in SLC52A2 have been associated with Brown-Vialetto-Van Laere syndrome 2 (BVVLS2) - an autosomal recessive progressive neurologic disorder characterized by deafness, bulbar dysfunction, and axial and limb hypotonia (PMID: 22864630, 23243084).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SLC52A2 antibody 30396-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |