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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC43A2 Polyclonal antibody (30031-1-AP)

    WB analysis using 30031-1-AP (same clone as 30031-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30031-1-AP (SLC43A2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30031-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SLC43A2 Polyclonal antibody (30031-1-AP)

    WB analysis using 30031-1-AP (same clone as 30031-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30031-1-AP (SLC43A2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30031-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using SLC43A2 Polyclonal antibody (30031-1-AP)

    IHC staining of human placenta using 30031-1-AP (same clone as 30031-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30031-1-AP (SLC43A2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30031-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using SLC43A2 Polyclonal antibody (30031-1-AP)

    IHC staining of human placenta using 30031-1-AP (same clone as 30031-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30031-1-AP (SLC43A2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30031-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using SLC43A2 Polyclonal antibody (30031-1-AP)

    IF Staining of human lung cancer using 30031-1-AP (same clone as 30031-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using SLC43A2 antibody (30031-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30031-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using SLC43A2 Polyclonal antibody (30031-1-AP)

    FC experiment of Jurkat using 30031-1-AP (same clone as 30031-1-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug SLC43A2 Polyclonal antibody (30031-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00017). This data was developed using the same antibody clone with 30031-1-PBS in a different storage buffer formulation.

SLC43A2 Polyclonal antibody, PBS Only

SLC43A2 Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA
Cat No. 30031-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Synonyms

LAT4, L-type amino acid transporter 4, PP7664

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30031-1-PBS targets SLC43A2 in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag31341

Product name: Recombinant human SLC43A2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 516-569 aa of BC027923

Sequence: WVNVGLLLLSLLGFCLPLYLICYRRQLERQLQQRQEDDKLFLKINGSSNQEAFV

相同性解析による交差性が予測される生物種
Full Name solute carrier family 43, member 2
Calculated molecular weight 63 kDa
Observed molecular weight 48-53 kDa
GenBank accession numberBC027923
Gene Symbol SLC43A2
Gene ID (NCBI) 124935
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N370
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SLC43A2 (solute carrier family 43 member 2, also known as LAT4) is a multi-pass transmembrane protein that mediates sodium- and chloride-independent uptake of large neutral amino acids, including methionine, leucine and phenylalanine. Highly expressed in kidney, placenta and liver, it supplies essential amino acids to support fetal growth, protein synthesis and one-carbon metabolism. Over-expressed in head-and-neck, liver and other cancers, SLC43A2-driven methionine influx maintains methylation reactions and YAP activity, promoting tumor proliferation and chemo-resistance . Pharmacological inhibition of SLC43A2 sensitizes tumors to therapy, highlighting its potential as a metabolic target. (PMID: 39747898; 15659399; 30379325)

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