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Validation Data Gallery

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  • Western Blot (WB) analysis of Caco-2 cells using SLC38A7 Recombinant antibody (83346-6-RR)

    WB analysis of Caco-2 using 83346-6-RR (same clone as 83346-6-PBS)

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 83346-6-RR (SLC38A7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83346-6-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using SLC38A7 Recombinant antibody (83346-6-RR)

    WB analysis of human liver using 83346-6-RR (same clone as 83346-6-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 83346-6-RR (SLC38A7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83346-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Caco-2 cells using SLC38A7 Recombinant antibody (83346-6-RR)

    FC experiment of Caco-2 using 83346-6-RR (same clone as 83346-6-PBS)

    1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug SLC38A7 Recombinant antibody (83346-6-RR, Clone:240147C3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83346-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U-251 cells using SLC38A7 Recombinant antibody (83346-6-RR)

    FC experiment of U-251 using 83346-6-RR (same clone as 83346-6-PBS)

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug SLC38A7 Recombinant antibody (83346-6-RR, Clone:240147C3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83346-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83346-6-RR

    Affinity and Kinetic Characterization of 83346-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83346-6-RR against Human SLC38A7 were performed. The affinity constant is 1.31 nM.

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SLC38A7 Recombinant antibody, PBS Only

SLC38A7 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83346-6-PBS
Clone No.240147C3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

SNAT7, Solute carrier family 38 member 7, Sodium-coupled neutral amino acid transporter 7, FLJ12724, FLJ10815

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83346-6-PBS targets SLC38A7 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SLC38A7 fusion protein Ag35081 相同性解析による交差性が予測される生物種
Full Name solute carrier family 38, member 7
Calculated molecular weight 50 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC001961
Gene Symbol SLC38A7
Gene ID (NCBI) 55238
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9NVC3
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SLC38A7 also known as SNAT7, is a member of the SLC38 family that encodes sodium-coupled neutral amino acid transporters. SLC38A7 is a system N transporter that has a substrate preference for L-glutamine. It also transports other amino acids with polar side chains, as well as L-histidine and L-alanine (PMID: 21511949).