Validation Data Gallery
Tested Applications
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31486-1-AP targets SLC38A7 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag35081 Product name: Recombinant human SLC38A7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-53 aa of BC001961 Sequence: MAQVSINNDYSEWDLSTDAGERARLLQSPCVDTAPKSEWEASPGGLDRGTTST 相同性解析による交差性が予測される生物種 |
| Full Name | solute carrier family 38, member 7 |
| Calculated molecular weight | 50 kDa |
| GenBank accession number | BC001961 |
| Gene Symbol | SLC38A7 |
| Gene ID (NCBI) | 55238 |
| RRID | AB_3670003 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9NVC3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SLC38A7 also known as SNAT7, is a system N transporter with a substrate preference for L-glutamine(PMID: 21511949). SLC38A7 symporter that selectively transports sodium ions and amino acids, such as L-glutamine and L-asparagine from the lysosome into the cytoplasm and may participate in mTORC1 activation (PubMed:28416685, PubMed:35561222). colocalization of Slc38a7 with neuronal markers in excitatory and inhibitory neurons, but not in astrocytes or glial cells.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SLC38A7 antibody 31486-1-AP | Download protocol |
| IHC protocol for SLC38A7 antibody 31486-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |