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Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using SLC38A6 Polyclonal antibody (27636-1-AP)

    WB analysis of SH-SY5Y using 27636-1-AP (same clone as 27636-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 27636-1-AP (SLC38A6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27636-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using SLC38A6 Polyclonal antibody (27636-1-AP)

    WB analysis of SH-SY5Y using 27636-1-AP (same clone as 27636-1-PBS)

    WB result of SLC38A6 antibody (27636-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC38A6 transfected SH-SY5Y cells. This data was developed using the same antibody clone with 27636-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SLC38A6 Polyclonal antibody (27636-1-AP)

    IHC staining of mouse brain using 27636-1-AP (same clone as 27636-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27636-1-AP (SLC38A6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27636-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SLC38A6 Polyclonal antibody (27636-1-AP)

    IHC staining of mouse brain using 27636-1-AP (same clone as 27636-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27636-1-AP (SLC38A6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27636-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SLC38A6 Polyclonal antibody (27636-1-AP)

    IF Staining of mouse brain using 27636-1-AP (same clone as 27636-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SLC38A6 antibody (27636-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27636-1-PBS in a different storage buffer formulation.

SLC38A6 Polyclonal antibody, PBS Only

SLC38A6 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 27636-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Amino acid transporter SLC38A6, NAT1, NAT-1, N-system amino acid transporter 1, SNAT6

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27636-1-PBS targets SLC38A6 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag24654

Product name: Recombinant human SLC38A6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 142-250 aa of BC110378

Sequence: IIKTELPAAIAEFLTGDYSRYWYLDGQTLLIIICVGIVFPLALLPKIGFLGYTSSLSFFFMMFFALVVIIKKWSIPCPLTLNYVEKGFQISNVTDDCKPKLFHFSKESA

相同性解析による交差性が予測される生物種
Full Name solute carrier family 38, member 6
Calculated molecular weight 456 aa, 51 kDa
Observed molecular weight43 kDa
GenBank accession numberBC110378
Gene Symbol SLC38A6
Gene ID (NCBI) 145389
RRIDAB_3085978
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IZM9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SLC38A6 is also known as SNAT6 and belongs to the amino acid/polyamine transporter 2 family. SLC38A6 is probably a sodium-dependent amino acid/proton antiporter or a neuronal transporter for glutamate. SLC38A6 has 2 isoforms with the molecular mass of 51 kDa and 58 kDa. The observed molecular weight of SLC38A6 is 43 kDa, which is consistent with the description in the literature of MW between 37 kDa and 50 kDa (PMID: 24752331).

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