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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC38A3 Polyclonal antibody (14315-1-AP)

    WB analysis using 14315-1-AP (same clone as 14315-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14315-1-AP (SLC38A3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14315-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using SLC38A3 Polyclonal antibody (14315-1-AP)

    WB analysis of mouse liver using 14315-1-AP (same clone as 14315-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 14315-1-AP (SLC38A3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14315-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse pancreas tissue using SLC38A3 Polyclonal antibody (14315-1-AP)

    WB analysis of mouse pancreas using 14315-1-AP (same clone as 14315-1-PBS)

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14315-1-AP (SLC38A3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14315-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using SLC38A3 Polyclonal antibody (14315-1-AP)

    IP experiment of mouse heart using 14315-1-AP (same clone as 14315-1-PBS)

    IP result of anti-SLC38A3 (IP:14315-1-AP, 3ug; Detection:14315-1-AP 1:1000) with mouse heart tissue lysate 4000ug. This data was developed using the same antibody clone with 14315-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SLC38A3 Polyclonal antibody (14315-1-AP)

    IHC staining of human lung cancer using 14315-1-AP (same clone as 14315-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 14315-1-AP (SLC38A3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14315-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SLC38A3 Polyclonal antibody (14315-1-AP)

    IHC staining of human lung cancer using 14315-1-AP (same clone as 14315-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 14315-1-AP (SLC38A3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14315-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SLC38A3 Polyclonal antibody (14315-1-AP)

    IF Staining of HEK-293 using 14315-1-AP (same clone as 14315-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 14315-1-AP (SLC38A3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14315-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SLC38A3 Polyclonal antibody, PBS Only

SLC38A3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14315-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14315-1-PBS targets SLC38A3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5553

Product name: Recombinant human SLC38A3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-191 aa of BC042875

Sequence: MEAPLQTEMVELVPNGKHSEGLLPVITPMAGNQRVEDPARSCMEGKSFLQKSPSKEPHFTDFEGKTSFGMSVFNLSNAIMGSGILGLAYAMANTGIILFLFLLTAVALLSSYSIHLLLKSSGVVGIRAYEQLGYRAFGTPGKLAAALAITLQNIGAMSSYLYIIKSELPLVIQTFLNLEEKTSDWYMNGNY

相同性解析による交差性が予測される生物種
Full Name solute carrier family 38, member 3
Calculated molecular weight 504 aa, 56 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC042875
Gene Symbol SLC38A3
Gene ID (NCBI) 10991
RRIDAB_2254879
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99624
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SLC38A3, also known as SNAT3 or SN1, belongs to the SLC38 family of sodium-coupled neutral amino acid transporters. SLC38A3 mediates glutamine transport with sodium and proton coupled manner, and it contributes to ammonia metabolism in the liver and brain. SLC38A3 is highly expressed in the liver and brain astrocytes, with modest expression in the kidney, heart, and muscle. SLC38A3 may migrate higher than the predicted 56 kDa due to the glycosylation.

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