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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using SLC36A4 Polyclonal antibody (24066-1-AP)

    WB analysis of A549 using 24066-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 24066-1-AP (SLC36A4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SLC36A4 Polyclonal antibody (24066-1-AP)

    WB analysis of HEK-293 using 24066-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24066-1-AP (SLC36A4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using SLC36A4 Polyclonal antibody (24066-1-AP)

    WB analysis of PC-3 using 24066-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 24066-1-AP (SLC36A4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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SLC36A4 Polyclonal antibody

SLC36A4 Polyclonal Antibody for WB, ELISA
Cat No. 24066-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

PAT4, SLC36A4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24066-1-AP targets SLC36A4 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC36A4 fusion protein Ag21142 相同性解析による交差性が予測される生物種
Full Name solute carrier family 36 (proton/amino acid symporter), member 4
Calculated molecular weight 504 aa, 56 kDa
Observed molecular weight 41-66 kDa
GenBank accession numberBC047374
Gene Symbol SLC36A4
Gene ID (NCBI) 120103
RRIDAB_2879424
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ6YBV0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC36A4 (also known as proton-coupled amino-acid transporter 4, PAT4), is a member of the solute carrier (SLC) 36 family which functions as transporter for amino acids and derivatives. SLC36A4 is widely distributed at the mRNA level in normal tissues and many cancer cell lines. It modulates the mTORC1 signaling and is essential for the proliferation of cancer cells. In addition, small GTPase Rab12 controls the degradation of SLC36A4, which indirectly modulates mTORC1 activity and autophagy. This antibody can recognize the endogenous SLC36A4 protein in multiple cell lines.

Protocols

Product Specific Protocols
WB protocol for SLC36A4 antibody 24066-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols