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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC28A3 Polyclonal antibody (18182-1-AP)

    WB analysis using 18182-1-AP (same clone as 18182-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18182-1-AP (SLC28A3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18182-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SLC28A3 Polyclonal antibody (18182-1-AP)

    IHC staining of human pancreas cancer using 18182-1-AP (same clone as 18182-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 18182-1-AP (SLC28A3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18182-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SLC28A3 Polyclonal antibody (18182-1-AP)

    IHC staining of human pancreas cancer using 18182-1-AP (same clone as 18182-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 18182-1-AP (SLC28A3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18182-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SLC28A3 Polyclonal antibody (18182-1-AP)

    IF Staining of HepG2 using 18182-1-AP (same clone as 18182-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using SLC28A3 antibody (18182-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18182-1-PBS in a different storage buffer formulation.

SLC28A3 Polyclonal antibody, PBS Only

SLC28A3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 18182-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18182-1-PBS targets SLC28A3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12887

Product name: Recombinant human SLC28A3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-186 aa of BC093821

Sequence: MELRSTAAPRAEGYSNVGFQNEENFLENENTSGNNSIRSRAVQSREHTNTKQDEEQVTVEQDSPRNREHMEDDDEEMQQKGCLERRYDTVCGFCRKHKTTLRHIIWGILLAGYLVMVISACVLNFHRALPLFVITVAAIFFVVWDHLMAKYEHRIDEMLSPGRRLLNSHWFWLKWVIWSSLVLAVI

相同性解析による交差性が予測される生物種
Full Name solute carrier family 28 (sodium-coupled nucleoside transporter), member 3
Calculated molecular weight 691 aa, 77 kDa
Observed molecular weight70-75 kDa
GenBank accession numberBC093821
Gene Symbol SLC28A3
Gene ID (NCBI) 64078
RRIDAB_3085558
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HAS3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SLC28A3 (Solute carrier family 28 member 3), also named CNT3, has sodium-dependent, pyrimidine- and purine-selective (PMID: 11032837, PMID: 15861042, PMID: 16446384, PMID: 17140564, PMID: 21998139). It is involved in the homeostasis of endogenous nucleosides (PMID: 11032837, PMID: 15861042). SLC28A3 is able to transports uridine, gemcitabine, 3'-azido-3'-deoxythymidine (AZT), ribavirin and 3-deazauridine (PMID: 21795683, PMID: 11032837, PMID: 17140564). SLC28A3, the human concentrative nucleoside transporter, plays a role in mediating the cellular entry of a broad array of physiological nucleosides and synthetic anticancer nucleoside analog drugs (PMID: 15738947). Variants in SLC28A3 and UGT1A6 act as genetic markers predictive of anthracycline-induced cardiotoxicity (PMID: 23441093).

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