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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC26A5 Polyclonal antibody (22013-1-AP)

    WB analysis using 22013-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22013-1-AP (SLC26A5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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SLC26A5 Polyclonal antibody

SLC26A5 Polyclonal Antibody for WB, ELISA
Cat No. 22013-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Prestin, PRES, DFNB61

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inmouse brain tissue, mouse heart tissue, rat brain tissue, rat heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22013-1-AP targets SLC26A5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC26A5 fusion protein Ag16631 相同性解析による交差性が予測される生物種
Full Name solute carrier family 26, member 5 (prestin)
Calculated molecular weight 744 aa, 81 kDa
Observed molecular weight70 kDa
GenBank accession numberBC100832
Gene Symbol SLC26A5
Gene ID (NCBI) 375611
RRIDAB_3669384
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP58743
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC26A5 (Prestin) is a protein that is housed in abundance within the lateral membrane of outer hair cells (OHC) in the organ of Corti. The protein imparts robust electromechanical activity to the cell that is unlike any other form of cellular motility, notably associated with a voltage-dependent, bell-shaped nonlinear capacitance (NLC) that reports on conformational changes in the protein (PMID: 32061780). SLC26A5 is responsible for acute sensitivity and frequency selectivity in the vertebrate auditory system (PMID: 34294052).

Protocols

Product Specific Protocols
WB protocol for SLC26A5 antibody 22013-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols