Validation Data Gallery
Tested Applications
| Positive WB detected in | HCT 116 cells, Caco-2 cells, COLO 205 cells, HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87216-1-RR targets SLC26A3 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag34479 Product name: Recombinant human SLC26A3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 497-764 aa of BC025671 Sequence: LTIVFRTQFPKCSTLANIGRTNIYKNKKDYYDMYEPEGVKIFRCPSPIYFANIGFFRRKLIDAVGFSPLRILRKRNKALRKIRKLQKQGLLQVTPKGFICTVDTIKDSDEELDNNQIEVLDQPINTTDLPFHIDWNDDLPLNIEVPKISLHSLILDFSAVSFLDVSSVRGLKSILQEFIRIKVDVYIVGTDDDFIEKLNRYEFFDGEVKSSIFFLTIHDAVLHILMKKDYSTSKFNPSQEKDGKIDFTINTNGGLRNRVYEVPVETKF 相同性解析による交差性が予測される生物種 |
| Full Name | solute carrier family 26, member 3 |
| Calculated molecular weight | 764 aa, 85 kDa |
| Observed molecular weight | 85 kDa |
| GenBank accession number | BC025671 |
| Gene Symbol | SLC26A3 |
| Gene ID (NCBI) | 1811 |
| RRID | AB_3745411 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P40879 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SLC26A3 (Chloride anion exchanger), also known as DRA. It is expected to be located in the cytoplasm and cell membranes. It is a transmembrane glycoprotein, which is mainly located in the lower digestive tract mucosa, especially in the apical membrane of columnar epithelium and some goblet cells. The expression level of SLC26A3 in intestine is different along different parts of intestine, and there are also differences on crypt-villus axis. It is mainly expressed in the apical membrane of colon epithelial cells, less in jejunum and ileum, but not in esophagus. It participates in the absorption of electrically neutral NaCl in intestine and works with Na+/H+ exchanger. Cl-/HCO3- exchange mediated by SLC26A3 is very important for maintaining intestinal acid-base balance. In duodenum, the physiological function of SLC26A3 is related to HCO3- secretion, which plays a central role in neutralizing gastric acid (PMID: 32989468). The molecular weight of SLC26A3 is 84 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC26A3 antibody 87216-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |