Validation Data Gallery
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Tested Applications
| Positive WB detected in | L02 cells, HEK-293 cells, human liver tissue, human heart tissue, human placenta tissue, HEK-293T cells |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human skeletal muscle tissue, human heart tissue, human kidney tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 7 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
| CoIP | See 2 publications below |
Product Information
14841-1-AP targets SLC25A6-Specific in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 6 |
| Calculated molecular weight | 33 kDa |
| Observed molecular weight | 30-33 kDa |
| GenBank accession number | NM_001636 |
| Gene Symbol | SLC25A6 |
| Gene ID (NCBI) | 293 |
| RRID | AB_2190371 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P12236 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SLC25A6, also known as ANT3 (adenine nucleotide translocase 3), is a member of the solute carrier family 25, specifically the mitochondrial carrier subfamily. As part of the mitochondrial carrier family, SLC25A6 plays a role in the mitochondrial energy production process by facilitating the exchange of metabolites necessary for oxidative phosphorylation (PMID: 23506884).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC25A6-Specific antibody 14841-1-AP | Download protocol |
| IHC protocol for SLC25A6-Specific antibody 14841-1-AP | Download protocol |
| IF protocol for SLC25A6-Specific antibody 14841-1-AP | Download protocol |
| IP protocol for SLC25A6-Specific antibody 14841-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Curr Biol Defining the Substrate Spectrum of the TIM22 Complex Identifies Pyruvate Carrier Subunits as Unconventional Cargos. | ||
Am J Cancer Res PTPMT1 regulates mitochondrial death through the SLC25A6-NDUFS2 axis in pancreatic cancer cells | ||
Hum Mol Genet Mutations of the mitochondrial carrier translocase channel subunit TIM22 cause early-onset mitochondrial myopathy. | ||
Cancer Cell Int Discovery and validation of FBLN1 and ANT3 as potential biomarkers for early detection of cervical cancer. | ||
FEBS Lett TIM29 is a subunit of the human carrier translocase required for protein transport. | ||
iScience MRPL12-ANT3 interaction involves in acute kidney injury via regulating MPTP of tubular epithelial cells |