Validation Data Gallery
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Tested Applications
| Positive WB detected in | Jurkat cells, mouse brain tissue, rat brain tissue, MCF-7 cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 9 publications below |
| IF | See 1 publications below |
Product Information
12277-1-AP targets SLC25A46 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2923 Product name: Recombinant human SLC25A46 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-264 aa of BC017169 Sequence: MHPRRPDGFDGLGYRGGARDEQGFGGAFPARSFSTGSDLGHWVTTPPDIPGSRNLHWGEKSPPYGVPTTSTPYEGPTEEPFSSGGGGSVQGQSSEQLNRFAGFGIGLASLFTENVLAHPCIVLRRQCQVNYHAQHYHLTPFTVINIMYSFNKTQGPRALWKGMGSTFIVQGVTLGAEGIISEFTPLPREVLHKWSPKQIGEHLLLKSLTYVVAMPFYSASLIETVQSEIIRDNTGILECVKEGIGRVIGMGVPHSKRLLPLLSL 相同性解析による交差性が予測される生物種 |
| Full Name | solute carrier family 25, member 46 |
| Calculated molecular weight | 418 aa, 46 kDa |
| Observed molecular weight | 46 kDa |
| GenBank accession number | BC017169 |
| Gene Symbol | SLC25A46 |
| Gene ID (NCBI) | 91137 |
| RRID | AB_2239384 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96AG3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SLC25A46 belongs to the SLC25 family of mitochondrial carrier proteins.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SLC25A46 antibody 12277-1-AP | Download protocol |
| WB protocol for SLC25A46 antibody 12277-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1. | ||
EMBO Mol Med SLC25A46 is required for mitochondrial lipid homeostasis and cristae maintenance and is responsible for Leigh syndrome.
| ||
J Pers Med Reanalysis of Exome Data Identifies Novel SLC25A46 Variants Associated with Leigh Syndrome. | ||
Mol Biol Cell Rapid degradation of mutant SLC25A46 by the ubiquitin-proteasome system results in MFN1/2 mediated hyperfusion of mitochondria.
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