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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using SLC25A45 Polyclonal antibody (14160-1-AP)

    WB analysis of mouse skeletal muscle using 14160-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14160-1-AP (SLC25A45 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using SLC25A45 Polyclonal antibody (14160-1-AP)

    WB analysis of SH-SY5Y using 14160-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14160-1-AP (SLC25A45 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using SLC25A45 Polyclonal antibody (14160-1-AP)

    IF Staining of SH-SY5Y using 14160-1-AP

    Immunofluorescent analysis of SH-SY5Y cells using 14160-1-AP (SLC25A45 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

SLC25A45 Polyclonal antibody

SLC25A45 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 14160-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Solute carrier family 25 member 45

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, SH-SY5Y cells
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14160-1-AP targets SLC25A45 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5342

Product name: Recombinant human SLC25A45 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 97-335 aa of BC041100

Sequence: DLIKVRLQNQTEPRAQPGSPPPRYQGPVHCAASIFREEGPRGLFRGAWALTLRDTPTVGIYFITYEGLCRQYTPEGQNPSSATVLVAGGLCRHCFLGGSHALRRDQVPDADGWTETQSVPGDAGLHGEQHPAGRTGSLLPGGHHQQCPRLSRQCCHLPQLRISPPLVGMSPAAMPAAPHQAHGLEASLRLEARLKACKSVQEAQPFLTKVPPTRADLGWADTCGSRKPGACAASLCSWP

相同性解析による交差性が予測される生物種
Full Name solute carrier family 25, member 45
Calculated molecular weight 335 aa, 36 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC041100
Gene Symbol SLC25A45
Gene ID (NCBI) 283130
RRIDAB_2190339
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N413
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for SLC25A45 antibody 14160-1-APDownload protocol
WB protocol for SLC25A45 antibody 14160-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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