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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC25A40 Polyclonal antibody (20615-1-AP)

    WB analysis using 20615-1-AP (same clone as 20615-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20615-1-AP (SLC25A40 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20615-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using SLC25A40 Polyclonal antibody (20615-1-AP)

    IHC staining of human placenta using 20615-1-AP (same clone as 20615-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20615-1-AP (SLC25A40 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20615-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using SLC25A40 Polyclonal antibody (20615-1-AP)

    IHC staining of human placenta using 20615-1-AP (same clone as 20615-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20615-1-AP (SLC25A40 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20615-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SLC25A40 Polyclonal antibody (20615-1-AP)

    IHC staining of mouse kidney using 20615-1-AP (same clone as 20615-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20615-1-AP (SLC25A40 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20615-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SLC25A40 Polyclonal antibody (20615-1-AP)

    IHC staining of mouse kidney using 20615-1-AP (same clone as 20615-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20615-1-AP (SLC25A40 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20615-1-PBS in a different storage buffer formulation.

SLC25A40 Polyclonal antibody, PBS Only

SLC25A40 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 20615-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

MCFP

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20615-1-PBS targets SLC25A40 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14669

Product name: Recombinant human SLC25A40 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-110 aa of BC027322

Sequence: MDPETRGQEIIKVTPLQQMLASCTGAILTSVIVTPLDVVKIRLQAQNNPLPKGKCFVYSNGLMDHLCVCEEGGNKLWYKKPGNFQGTLDAFFKIIRNEGIKSLWSGLPPT

相同性解析による交差性が予測される生物種
Full Name solute carrier family 25, member 40
Calculated molecular weight 338 aa, 38 kDa
Observed molecular weight30~38 kDa
GenBank accession numberBC027322
Gene Symbol SLC25A40
Gene ID (NCBI) 55972
RRIDAB_3669338
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TBP6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SLC25A40 (solute carrier family 25, member 40) is one of the solute carrier (SLC) transporters that comprise a family of MCS transporters and uptakes GSH into mitochondria (PMID: 37407483). The SLC25A40 mRNA levels were significantly increased in both the kidneys of LPS mice and KMRC-1 cells after LPS treatment, when compared with those in the controls (PMID: 35955707).

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