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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC25A31/ANT4 Polyclonal antibody (33263-1-AP)

    WB analysis using 33263-1-AP (same clone as 33263-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 33263-1-AP (ANT4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. Negative control: mouse brain tissue, mouse heart tissue. This data was developed using the same antibody clone with 33263-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SLC25A31/ANT4 Polyclonal antibody (33263-1-AP)

    IHC staining of mouse testis using 33263-1-AP (same clone as 33263-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 33263-1-AP (SLC25A31/ANT4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33263-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SLC25A31/ANT4 Polyclonal antibody (33263-1-AP)

    IHC staining of mouse testis using 33263-1-AP (same clone as 33263-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 33263-1-AP (SLC25A31/ANT4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33263-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SLC25A31/ANT4 Polyclonal antibody (33263-1-AP)

    IHC staining of mouse kidney using 33263-1-AP (same clone as 33263-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 33263-1-AP (SLC25A31/ANT4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33263-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SLC25A31/ANT4 Polyclonal antibody (33263-1-AP)

    IHC staining of mouse kidney using 33263-1-AP (same clone as 33263-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 33263-1-AP (SLC25A31/ANT4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33263-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using SLC25A31/ANT4 Polyclonal antibody (33263-1-AP)

    IF Staining of U-251 using 33263-1-AP (same clone as 33263-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using SLC25A31/ANT4 antibody (33263-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 33263-1-PBS in a different storage buffer formulation.

SLC25A31/ANT4 Polyclonal antibody, PBS Only

SLC25A31/ANT4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 33263-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ANT4, SLC25A31, AAC4, Adenine nucleotide translocator 4, ADP,ATP carrier protein 4

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

33263-1-PBS targets SLC25A31/ANT4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11008

Product name: Recombinant human SLC25A31 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-315 aa of BC022032

Sequence: MHREPAKKKAEKRLFDASSFGKDLLAGGVAAAVSKTAVAPIERVKLLLQVQASSKQISPEARYKGMVDCLVRIPREQGFFSFWRGNLANVIRYFPTQALNFAFKDKYKQLFMSGVNKEKQFWRWFLANLASGGAAGATSLCVVYPLDFARTRLGVDIGKGPEERQFKGLGDCIMKIAKSDGIAGLYQGFGVSVQGIIVYRASYFGAYDTVKGLLPKPKKTPFLVSFFIAQVVTTCSGILSYPFDTVRRRMMMQSGEAKRQYKGTLDCFVKIYQHEGISSFFRGAFSNVLRGTGGALVLVLYDKIKEFFHIDIGGR

相同性解析による交差性が予測される生物種
Full Name solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 31
Calculated molecular weight 315 aa, 35 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC022032
Gene Symbol SLC25A31
Gene ID (NCBI) 83447
RRIDAB_3742919
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9H0C2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SLC25A31, also known as ANT4, is a member of the mitochondrial carrier family. The proteins of this family are mainly involved in the transport of substances inside and outside the mitochondria. SLC25A31 is an ADP/ATP antiporter protein, which catalyzes the exchange of ATP generated in mitochondria by ATP synthase against ADP produced in cytosol by most energy-consuming reactions(PMID: 15670820). SLC25A31 is mainly expressed in the testis and male germ cells (PMID: 25031318, 23506884).

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