Validation Data Gallery

Filter: AllWBIHC

WB analysis of HeLa using 10420-1-AP
WB result of SLC25A3 antibody (10420-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC25A3 transfected HeLa cells.
WB analysis using 10420-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10420-1-AP (SLC25A3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 10420-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10420-1-AP (SLC25A3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 10420-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10420-1-AP (SLC25A3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat liver using 10420-1-AP
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 10420-1-AP (SLC25A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 10420-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10420-1-AP (SLC25A3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

SLC25A3 Polyclonal antibody

SLC25A3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 10420-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

PiC, PTP, Phosphate transport protein, PHC, OK/SW-cl.48

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (10)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, HepG2 cells, HeLa cells, MOLT-4 cells, PC-12 cells
Positive IHC detected in	human lung cancer tissue, rat liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 6 publications below
IHC	See 1 publications below
IF	See 2 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

10420-1-AP targets SLC25A3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity	human, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0667 Product name: Recombinant human SLC25A3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-361 aa of BC003504 Sequence: MFSSVAHLARANPFNTPHLQLVHDGLGDLRSSSPGPTGQPRRPRNLAAAAVEEYSCEFGSAKYYALCGFGGVLSCGLTHTAVVPLDLVKCRMQVDPQKYKGIFNGFSVTLKEDGVRGLAKGWAPTFLGYSMQGLCKFGFYEVFKVLYSNMLGEENTYLWRTSLYLAASASAEFFADIALAPMEAAKVRIQTQPGYANTLRDAAPKMYKEEGLKAFYKGVAPLWMRQIPYTMMKFACFERTVEALYKFVVPKPRSECSKPEQLVVTFVAGYIAGVFCAIVSHPADSVVSVLNKEKGSSASLVLKRLGFKGVWKGLFARIIMIGTLTALQWFIYDSVKVYFRLPRPPPPEMPESLKKKLGLTQ 相同性解析による交差性が予測される生物種
Full Name	solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 3
Calculated molecular weight	40 kDa
Observed molecular weight	40-43 kDa
GenBank accession number	BC003504
Gene Symbol	SLC25A3
Gene ID (NCBI)	5250
RRID	AB_2877735
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q00325
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The mitochondrial phosphate carrier SLC25A3 belongs to the SLC25 family , which is the largest family of solute carriers. SLC25A3 encodes the phosphate carrier PiC that catalyses the import of phosphate into mitochondria where it is needed for ATP synthesis. SLC25A3 has two isoforms SLC25A3-A (a heart-type isoform) and SLC25A3-B (a liver-type isoform) as a result of alternative splicing of exon 3. It has been reported that SLC25A3 mutations are associated with mitochondrial phosphate carrier deficiency, respiratory distress and hypertrophic cardiomyopathy. (PMID: 15984930, 27780865, 20877624)

Protocols

Product Specific Protocols
IHC protocol for SLC25A3 antibody 10420-1-AP	Download protocol
WB protocol for SLC25A3 antibody 10420-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Am J Respir Cell Mol Biol Transcriptomics Analysis Identifies the Decline in the AT2 Stem Cell Niche in Aged Human Lungs Authors - Xue Liu View Article
human	WB	Cell Rep Mitochondrial transplantation rescues Ca2+ homeostasis imbalance and myocardial hypertrophy in SLC25A3-related hypertrophic cardiomyopathy Authors - Shuang Li View Article
human,mouse	WB	Exp Gerontol METTL14-mediated methylation of SLC25A3 mitigates mitochondrial damage in osteoblasts, leading to the improvement of osteoporosis Authors - Ping Wang KD Validated View Article
human	WB,IP,IF	bioRxiv Endothelial Cu Uptake Transporter CTR1 Senses Disturbed Flow to Promote Atherosclerosis through Cuproptosis Authors - Varadarajan Sudhahar View Article
mouse	WB	Dev Cell Slc25a3-dependent copper transport controls flickering-induced Opa1 processing for mitochondrial safeguard Authors - Daisuke Murata View Article
human	WB	BMC Biol Organic cation transporter 3 on neuronal mitochondria mediates MPP+-induced mitochondrial dysfunction and neurotoxicity in a TIMM22-dependent manner Authors - Ao Guan View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 10420-1-AP

WB analysis using 10420-1-AP

WB analysis using 10420-1-AP

IHC staining of human lung cancer using 10420-1-AP

IHC staining of rat liver using 10420-1-AP

IHC staining of human lung cancer using 10420-1-AP

SLC25A3 Polyclonal antibody

SLC25A3 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Transcriptomics Analysis Identifies the Decline in the AT2 Stem Cell Niche in Aged Human Lungs

Mitochondrial transplantation rescues Ca2+ homeostasis imbalance and myocardial hypertrophy in SLC25A3-related hypertrophic cardiomyopathy

METTL14-mediated methylation of SLC25A3 mitigates mitochondrial damage in osteoblasts, leading to the improvement of osteoporosis

Endothelial Cu Uptake Transporter CTR1 Senses Disturbed Flow to Promote Atherosclerosis through Cuproptosis

Slc25a3-dependent copper transport controls flickering-induced Opa1 processing for mitochondrial safeguard

Organic cation transporter 3 on neuronal mitochondria mediates MPP+-induced mitochondrial dysfunction and neurotoxicity in a TIMM22-dependent manner