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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC25A25 Polyclonal antibody

SLC25A25 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21568-1-AP

Synonyms

APC3, KIAA1896, MCSC, MCSC3, PCSCL, RP11 395P17.4, SCAMC 2, SCAMC2, SLC25A25

Validation Data Gallery

Applications:
  • WB analysis of RAW264.7 using 21568-1-AP

    WB analysis of RAW264.7 using 21568-1-AP

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 21568-1-AP (SLC25A25 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 21568-1-AP

    IHC staining of human liver using 21568-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 21568-1-AP (SLC25A25 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver using 21568-1-AP

    IHC staining of human liver using 21568-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 21568-1-AP (SLC25A25 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inRAW 264.7 cells, RAW264.7 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 21568-1-AP is SLC25A25 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC25A25 fusion protein Ag16086 相同性解析による交差性が予測される生物種
Full Name solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25
Calculated molecular weight 469 aa, 53 kDa
Observed molecular weight 50 kDa, 100 kDa
GenBank accession numberBC103933
Gene symbol SLC25A25
Gene ID (NCBI) 114789
RRIDAB_10858638
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SLC25A25 antibody 21568-1-APDownload protocol
IHC protocol for SLC25A25 antibody 21568-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Proc Natl Acad Sci U S A

Transcriptome-wide analysis of PGC-1α-binding RNAs identifies genes linked to glucagon metabolic action.

Authors - Clint D J Tavares
View Article