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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC25A22 Polyclonal antibody (25402-1-AP)

    WB analysis using 25402-1-AP (same clone as 25402-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25402-1-AP (SLC25A22 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25402-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SLC25A22 Polyclonal antibody (25402-1-AP)

    WB analysis using 25402-1-AP (same clone as 25402-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25402-1-AP (SLC25A22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25402-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SLC25A22 Polyclonal antibody (25402-1-AP)

    WB analysis using 25402-1-AP (same clone as 25402-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25402-1-AP (SLC25A22 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25402-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SLC25A22 Polyclonal antibody (25402-1-AP)

    IHC staining of mouse brain using 25402-1-AP (same clone as 25402-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25402-1-AP (SLC25A22 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25402-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SLC25A22 Polyclonal antibody (25402-1-AP)

    IHC staining of mouse brain using 25402-1-AP (same clone as 25402-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25402-1-AP (SLC25A22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25402-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SLC25A22 Polyclonal antibody (25402-1-AP)

    IHC staining of human liver cancer using 25402-1-AP (same clone as 25402-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25402-1-AP (SLC25A22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25402-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SLC25A22 Polyclonal antibody (25402-1-AP)

    IHC staining of human liver cancer using 25402-1-AP (same clone as 25402-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25402-1-AP (SLC25A22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25402-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SLC25A22 Polyclonal antibody (25402-1-AP)

    IHC staining of human colon cancer using 25402-1-AP (same clone as 25402-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25402-1-AP (SLC25A22 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25402-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SLC25A22 Polyclonal antibody (25402-1-AP)

    IHC staining of human colon cancer using 25402-1-AP (same clone as 25402-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25402-1-AP (SLC25A22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25402-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SLC25A22 Polyclonal antibody (25402-1-AP)

    IF Staining of mouse brain using 25402-1-AP (same clone as 25402-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SLC25A22 antibody (25402-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25402-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SLC25A22 Polyclonal antibody (25402-1-AP)

    IF Staining of mouse brain using 25402-1-AP (same clone as 25402-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 25402-1-AP (SLC25A22 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25402-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of COS-7 cells using SLC25A22 Polyclonal antibody (25402-1-AP)

    IF Staining of COS-7 using 25402-1-AP (same clone as 25402-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed COS-7 cells using 25402-1-AP (SLC25A22 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25402-1-PBS in a different storage buffer formulation.

SLC25A22 Polyclonal antibody, PBS Only

SLC25A22 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 25402-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25402-1-PBS targets SLC25A22 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag19958

Product name: Recombinant human SLC25A22 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 128-188 aa of BC023545

Sequence: KIQLQDAGRIAAQRKILAAQGQLSAQGGAQPSVEAPAAPRPTATQLTRDLLRSRGIAGLYK

相同性解析による交差性が予測される生物種
Full Name solute carrier family 25 (mitochondrial carrier: glutamate), member 22
Calculated molecular weight 323 aa, 34 kDa
Observed molecular weight 30-34 kDa
GenBank accession numberBC023545
Gene Symbol SLC25A22
Gene ID (NCBI) 79751
RRIDAB_2880060
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H936
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SLC25A22 encodes a mitochondrial glutamate/H+ symporter with highly expression in brain, especially in astrocytes, where it controls glutamate uptake. The mutations of SLC25A22 are associated with the early/infantile epileptic encephalopathies. It has been shown that SLC25A22 plays a key role in promoting proliferation and migration in cancer, such as human colorectal cancer (CRC) and Gallbladder cancer (GBC). And there is a result that the expression of SLC25A22 in GBC was significantly higher than that in adjacent tissues. 25402-1-AP antibody is specific to SLC25A22.(PMID: 30814911, 25033742, 24596948)

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