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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC25A17 Monoclonal antibody (67635-1-Ig)

    WB analysis using 67635-1-Ig (same clone as 67635-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67635-1-Ig (SLC25A17 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67635-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U2OS cells using SLC25A17 Monoclonal antibody (67635-1-Ig)

    WB analysis of U2OS using 67635-1-Ig (same clone as 67635-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 67635-1-Ig (SLC25A17 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67635-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using SLC25A17 Monoclonal antibody (67635-1-Ig)

    WB analysis of A549 using 67635-1-Ig (same clone as 67635-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 67635-1-Ig (SLC25A17 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67635-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SLC25A17 Monoclonal antibody (67635-1-Ig)

    IHC staining of human liver cancer using 67635-1-Ig (same clone as 67635-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67635-1-Ig (SLC25A17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67635-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SLC25A17 Monoclonal antibody (67635-1-Ig)

    IHC staining of human liver cancer using 67635-1-Ig (same clone as 67635-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67635-1-Ig (SLC25A17 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67635-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SLC25A17 Monoclonal antibody (67635-1-Ig)

    IHC staining of human liver cancer using 67635-1-Ig (same clone as 67635-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67635-1-Ig (SLC25A17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67635-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SLC25A17 Monoclonal antibody (67635-1-Ig)

    IHC staining of human liver cancer using 67635-1-Ig (same clone as 67635-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67635-1-Ig (SLC25A17 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67635-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using SLC25A17 Monoclonal antibody (67635-1-Ig)

    IHC staining of human liver using 67635-1-Ig (same clone as 67635-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67635-1-Ig (SLC25A17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67635-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using SLC25A17 Monoclonal antibody (67635-1-Ig)

    IHC staining of human liver using 67635-1-Ig (same clone as 67635-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67635-1-Ig (SLC25A17 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67635-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SLC25A17 Monoclonal antibody (67635-1-Ig)

    IHC staining of mouse liver using 67635-1-Ig (same clone as 67635-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67635-1-Ig (SLC25A17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67635-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SLC25A17 Monoclonal antibody (67635-1-Ig)

    IHC staining of mouse liver using 67635-1-Ig (same clone as 67635-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67635-1-Ig (SLC25A17 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67635-1-PBS in a different storage buffer formulation.

SLC25A17 Monoclonal antibody, PBS Only

SLC25A17 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67635-1-PBS
Clone No.2D1D2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

2D1D2, PMP34

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67635-1-PBS targets SLC25A17 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag24456

Product name: Recombinant human SLC25A17 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 34-104 aa of BC012998

Sequence: RLRLQVDEKRKSKTTHMVLLEIIKEEGLLAPYRGWFPVISSLCCSNFVYFYTFNSLKALWVKGQHSTTGKD

相同性解析による交差性が予測される生物種
Full Name solute carrier family 25 (mitochondrial carrier; peroxisomal membrane protein, 34kDa), member 17
Calculated molecular weight 307 aa, 35 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC012998
Gene Symbol SLC25A17
Gene ID (NCBI) 10478
RRIDAB_2882836
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO43808
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SLC25A17 (solute carrier family 25 member 17), is a kind of peroxisomal transporter for multiple cofactors such as CoA, FAD, FMN and AMP. It May catalyze the transport of free CoA, FAD and NAD+ from the cytosol into the peroxisomal matrix by a counter-exchange mechanism. SLC25A17 is the only member of the mitochondrial carrier family localized to peroxisomal. (PMID: 22185573, 32266253)

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