Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of HeLa using 15317-1-AP (same clone as 15317-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 15317-1-AP (SLC25A15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15317-1-PBS in a different storage buffer formulation.
WB analysis of mouse liver using 15317-1-AP (same clone as 15317-1-PBS)
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15317-1-AP (SLC25A15 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15317-1-PBS in a different storage buffer formulation.
WB analysis of mouse stomach using 15317-1-AP (same clone as 15317-1-PBS)
mouse stomach tissue were subjected to SDS PAGE followed by western blot with 15317-1-AP (SLC25A15 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15317-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 15317-1-AP (same clone as 15317-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15317-1-AP (SLC25A15 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15317-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 15317-1-AP (same clone as 15317-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15317-1-AP (SLC25A15 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15317-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 15317-1-AP (same clone as 15317-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15317-1-AP (SLC25A15 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15317-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 15317-1-AP (same clone as 15317-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15317-1-AP (SLC25A15 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15317-1-PBS in a different storage buffer formulation.
IHC staining of human skin using 15317-1-AP (same clone as 15317-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15317-1-PBS in a different storage buffer formulation.
IHC staining of human skin using 15317-1-AP (same clone as 15317-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15317-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 15317-1-AP (same clone as 15317-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15317-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 15317-1-AP (same clone as 15317-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15317-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 15317-1-AP (same clone as 15317-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15317-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 15317-1-AP (same clone as 15317-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15317-1-PBS in a different storage buffer formulation.
IHC staining of human ovary using 15317-1-AP (same clone as 15317-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15317-1-PBS in a different storage buffer formulation.
IHC staining of human ovary using 15317-1-AP (same clone as 15317-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15317-1-PBS in a different storage buffer formulation.
IF Staining of MCF-7 using 15317-1-AP (same clone as 15317-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using SLC25A15 antibody (15317-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15317-1-PBS in a different storage buffer formulation.

SLC25A15 Polyclonal antibody, PBS Only

SLC25A15 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 15317-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15317-1-PBS targets SLC25A15 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7563 Product name: Recombinant human SLC25A15 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-301 aa of BC002702 Sequence: MKSNPAIQAAIDLTAGAAGGTACVLTGQPFDTMKVKMQTFPDLYRGLTDCCLKTYSQVGFRGFYKGTSPALIANIAENSVLFMCYGFCQQVVRKVAGLDKQAKLSDLQNAAAGSFASAFAALVLCPTELVKCRLQTMYEMETSGKIAKSQNTVWSVIKSILRKDGPLGFYHGLSSTLLREVPGYFFFFGGYELSRSFFASGRSKDELGPVPLMLSGGVGGICLWLAVYPVDCIKSRIQVLSMSGKQAGFIRTFLNVVKNEGITALYSGLKPTMIRAFPANGALFLAYEYSRKLMMNQLEAY 相同性解析による交差性が予測される生物種
Full Name	solute carrier family 25 (mitochondrial carrier; ornithine transporter) member 15
Calculated molecular weight	33 kDa
Observed molecular weight	30-33 kDa
GenBank accession number	BC002702
Gene Symbol	SLC25A15
Gene ID (NCBI)	10166
RRID	AB_2189758
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y619
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

SLC25A15 gene encodes a 301-amino-acid-long mitochondrial carrier protein termed ORC1., which catalyzes the transport of cytosolic ornithine into mitochondria in exchange for citrulline (PMID: 19242930). Some mutations in the SLC25A15 gene have been reported to break the ornithine cycle and be related with human hyperornithinemia, hyperammonemia, and homocitrullinuria syndrome (PMID: 19242930, 24721342). This antibody detected SLC25A15 at an apprarant molecular mass about 33 kDa.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 15317-1-AP (same clone as 15317-1-PBS)

WB analysis of mouse liver using 15317-1-AP (same clone as 15317-1-PBS)

WB analysis of mouse stomach using 15317-1-AP (same clone as 15317-1-PBS)

IHC staining of human liver cancer using 15317-1-AP (same clone as 15317-1-PBS)

IHC staining of human liver cancer using 15317-1-AP (same clone as 15317-1-PBS)

IHC staining of human liver cancer using 15317-1-AP (same clone as 15317-1-PBS)

IHC staining of human liver cancer using 15317-1-AP (same clone as 15317-1-PBS)

IHC staining of human skin using 15317-1-AP (same clone as 15317-1-PBS)

IHC staining of human skin using 15317-1-AP (same clone as 15317-1-PBS)

IHC staining of human brain using 15317-1-AP (same clone as 15317-1-PBS)

IHC staining of human brain using 15317-1-AP (same clone as 15317-1-PBS)

IHC staining of human lung using 15317-1-AP (same clone as 15317-1-PBS)

IHC staining of human lung using 15317-1-AP (same clone as 15317-1-PBS)

IHC staining of human ovary using 15317-1-AP (same clone as 15317-1-PBS)

IHC staining of human ovary using 15317-1-AP (same clone as 15317-1-PBS)

IF Staining of MCF-7 using 15317-1-AP (same clone as 15317-1-PBS)

SLC25A15 Polyclonal antibody, PBS Only

SLC25A15 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information

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