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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SLC25A13 Polyclonal antibody (10789-1-AP)

    IHC staining of human colon cancer using 10789-1-AP (same clone as 10789-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10789-1-AP (SLC25A13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10789-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SLC25A13 Polyclonal antibody (10789-1-AP)

    IHC staining of human colon cancer using 10789-1-AP (same clone as 10789-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10789-1-AP (SLC25A13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10789-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SLC25A13 Polyclonal antibody (10789-1-AP)

    IHC staining of human lung cancer using 10789-1-AP (same clone as 10789-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10789-1-AP (SLC25A13 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10789-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using SLC25A13 Polyclonal antibody (10789-1-AP)

    IF Staining of U2OS using 10789-1-AP (same clone as 10789-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SLC25A13 antibody (10789-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 10789-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using SLC25A13 Polyclonal antibody (10789-1-AP)

    IF Staining of U2OS using 10789-1-AP (same clone as 10789-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SLC25A13 antibody (10789-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 10789-1-PBS in a different storage buffer formulation.

SLC25A13 Polyclonal antibody, PBS Only

SLC25A13 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 10789-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

ARALAR2, ARALAR-related gene 2, Calcium-binding mitochondrial carrier protein Aralar2, Citrin, Electrogenic aspartate/glutamate antiporter SLC25A13, mitochondrial

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10789-1-PBS targets SLC25A13 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1242

Product name: Recombinant human SLC25A13 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 336-675 aa of BC006566

Sequence: SVAGAVGATAVYPIDLVKTRMQNQRSTGSFVGELMYKNSFDCFKKVLRYEGFFGLYRGLLPQLLGVAPEKAIKLTVNDFVRDKFMHKDGSVPLAAEILAGGCAGGSQVIFTNPLEIVKIRLQVAGEITTGPRVSALSVVRDLGFFGIYKGAKACFLRDIPFSAIYFPCYAHVKASFANEDGQVSPGSLLLAGAIAGMPAASLVTPADVIKTRLQVAARAGQTTYSGVIDCFRKILREEGPKALWKGAGARVFRSSPQFGVTLLTYELLQRWFYIDFGGVKPMGSEPVPKSRINLPAPNPDHVGGYKLAVATFAGIENKFGLYLPLFKPSVSTSKAIGGGP

相同性解析による交差性が予測される生物種
Full Name solute carrier family 25, member 13 (citrin)
Calculated molecular weight 74 kDa
GenBank accession numberBC006566
Gene Symbol SLC25A13
Gene ID (NCBI) 10165
RRIDAB_2189738
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UJS0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SLC25A13, also known as citrin, has been identified as a mitochondrial aspartate-glutamate carrier protein and may be implicated in metabolic compartmentation. It consists of 675 amino acid residues and harbors four EF-hands and six mitochondrial transmembranous (TM) spanners. Mutations in SLC25A13 gene cause adult-onset type II citrullinemia (CTLN2) and neonatal intrahepatic cholestasis (NICCD). The complete deletion or cleaved SLA25A13 proteins have been reported in patients with SLC25A13 mutation.

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